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![]() [Cover Caption] Other Issues: |
Contents: Volume 276, Issue 2
February 1999 [Index by Author]
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Cover: Composite image demonstrating luminal surface expression of the cystic fibrosis transmembrane conductance regulator (CFTR) in concentric cross sections of small arteries from human lung tissue. Surrounding the concentric array, CFTR is exhibited along the surface and in the cytoplasm of cultured primary endothelial cells derived from human umbilical vein or lung microvasculature. Cells were highlighted by immunofluorescence using antibodies that recognize different domains of CFTR, including NBD-1 (red), COOH terminus (red), regulatory domain (green), or first extracellular loop (green). Vascular tissue was probed with anti-CFTR antibodies specific for the nucleotide binding domain or the COOH terminus (green apical delineation). Cells and tissues were counterstained with the nuclear dye, Hoechst 33258 (blue). See related article by A. Tousson, B. A. Van Tine, A. P. Naren, G. M. Shaw, and L. M. Schwiebert. Am. J. Physiol. 275 (Cell Physiol. 44): C1555-C1564, 1998.
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