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GROWTH, DIFFERENTIATION, AND APOPTOSIS

TGF--regulated collagen type I accumulation: role of Src-based signals

¹Division of Nephrology and Hypertension, Department of Medicine, and ²Department of Physiology and Biophysics, School of Medicine, Case Western Reserve University and University Hospitals of Cleveland, Cleveland, Ohio

Submitted 7 July 2006 ; accepted in final form 21 November 2006

Transforming growth factor- (TGF-) stimulates myofibroblast transdifferentiation, leading to type I collagen accumulation and fibrosis. We investigated the function of Src in TGF--induced collagen I accumulation. In human mesangial cells, PTyr416 Src (activated Src) was 3.3-fold higher in TGF--treated cells than in controls. Src activation by TGF- was blocked by rottlerin and by a dominant negative mutant of protein kinase C (PKC), showing that TGF- activates Src by a PKC-based mechanism. Pharmacological inhibitors and a dominant negative Src mutant prevented the increase in collagen type I secretion in cells exposed to TGF-. Similarly, on-target Src small interference RNA (siRNA) prevented type I collagen secretion in response to TGF-, but off-target siRNA complexes had no effect. It is well established in mesangial cells that upregulation of type I collagen by TGF- requires extracellular signal-regulated kinase 1/2 (ERK1/2), and we found that activation of ERK1/2 by TGF- requires Src. In conclusion, these results suggest that stimulation of collagen type I secretion by TGF- requires a PKC-Src-ERK1/2 signaling motif.

mesangial cells; fibrosis; glomerulus; transforming growth factor-

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