HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: 291/1/C59    most recent 00433.2005v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (8) Citing Articles via Google Scholar Google Scholar Articles by Praetorius, J. Articles by Nielsen, S. Search for Related Content PubMed PubMed Citation Articles by Praetorius, J. Articles by Nielsen, S.

MEMBRANE TRANSPORTERS, ION CHANNELS, AND PUMPS

Distribution of sodium transporters and aquaporin-1 in the human choroid plexus

The Water and Salt Research Center, Institute of Anatomy, University of Aarhus, Aarhus, Denmark

Submitted 26 August 2005 ; accepted in final form 8 February 2006

The choroid plexus epithelium secretes electrolytes and fluid in the brain ventricular lumen at high rates. Several channels and ion carriers have been identified as likely mediators of this transport in rodent choroid plexus. This study aimed to map several of these proteins to the human choroid plexus. Immunoperoxidase-histochemistry was employed to determine the cellular and subcellular localization of the proteins. The water channel, aquaporin (AQP) 1, was predominantly situated in the apical plasma membrane domain, although distinct basolateral and endothelial immunoreactivity was also observed. The Na⁺-K⁺-ATPase ₁-subunit was exclusively localized apically in the human choroid plexus epithelial cells. Immunoreactivity for the Na⁺-K⁺-2Cl^– cotransporter, NKCC1, was likewise confined to the apical plasma membrane domain of the epithelium. The Cl^–/HCO₃^– exchanger, AE2, was localized basolaterally, as was the Na⁺-dependent Cl^–/HCO₃^– exchanger, NCBE, and the electroneutral Na⁺-HCO₃^– cotransporter, NBCn1. No immunoreactivity was found toward the Na⁺-dependent acid/base transporters NHE1 or NBCe2. Hence, the human choroid plexus epithelium displays an almost identical distribution pattern of water channels and Na⁺ transporters as the rat and mouse choroid plexus. This general cross species pattern suggests central roles for these transporters in choroid plexus functions such as cerebrospinal fluid production.

immunohistochemistry; metabolism; cerebrospinal fluid secretion

This article has been cited by other articles:

				M. J. Wiegman, L. V. Bullinger, M. M. Kohlmeyer, T. C. Hunter, and M. J. Cipolla Regional Expression of Aquaporin 1, 4, and 9 in the Brain During Pregnancy Reproductive Sciences, May 1, 2008; 15(5): 506 - 516. [Abstract] [PDF]

				E. Boedtkjer, J. Praetorius, E.-M. Fuchtbauer, and C. Aalkjaer Antibody-independent localization of the electroneutral Na+-HCO3- cotransporter NBCn1 (slc4a7) in mice Am J Physiol Cell Physiol, February 1, 2008; 294(2): C591 - C603. [Abstract] [Full Text] [PDF]

				H. H. Damkier, S. Nielsen, and J. Praetorius Molecular expression of SLC4-derived Na+-dependent anion transporters in selected human tissues Am J Physiol Regulatory Integrative Comp Physiol, November 1, 2007; 293(5): R2136 - R2146. [Abstract] [Full Text] [PDF]

				A. J. Yool Aquaporins: Multiple Roles in the Central Nervous System Neuroscientist, October 1, 2007; 13(5): 470 - 485. [Abstract] [PDF]

				B. Banizs, P. Komlosi, M. O. Bevensee, E. M. Schwiebert, P. D. Bell, and B. K. Yoder Altered pHi regulation and Na+/HCO3- transporter activity in choroid plexus of cilia-defective Tg737orpk mutant mouse Am J Physiol Cell Physiol, April 1, 2007; 292(4): C1409 - C1416. [Abstract] [Full Text] [PDF]

				C. J. Taylor, P. A. Nicola, S. Wang, M. A. Barrand, and S. B. Hladky Transporters involved in regulation of intracellular pH in primary cultured rat brain endothelial cells J. Physiol., November 1, 2006; 576(3): 769 - 785. [Abstract] [Full Text] [PDF]