| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
RECEPTORS AND SIGNAL TRANSDUCTION
2-adrenoreceptor and the BKCa channel to uterine quiescence
Center for Reproduction and Early Life, Institute of Clinical Research, University of Nottingham, Nottingham NG7 2RD, United Kingdom
Submitted 12 May 2004 ; accepted in final form 17 August 2004
The 
2-adrenergic receptor (2-AR) and the large-conductance Ca2+-activated K+ (BKCa) channel have been shown, separately, to be involved in mediating uterine relaxation. Our recent studies reveal that the levels of both 2-AR and BKCa channel proteins in pregnant human myometrium decrease by 
50% after the onset of labor. We present direct evidence in support of a structural and functional association between the 2-AR and the BKCa channel in pregnant human myometrium. Localization of both proteins is predominantly plasmalemmal, with 60% of 2-AR colocalizing with the BKCa channel. Coimmunoprecipitation studies indicate that BKCa and 2-AR are structurally linked by direct protein-protein interactions. Functional correlation was confirmed by experiments of human myometrial contractility in which the BKCa channel blocker, paxilline, significantly antagonized the relaxant effect of the 2-AR agonist ritodrine. These novel findings provide an insight into the coupling between the 2-AR and BKCa channel and may have utility in the application of this signaling cascade for therapeutic potential in the management of preterm labor.
2-adrenergic receptor; myometrium; potassium channel; preterm labor; uterine contraction
This article has been cited by other articles:
|
|
 |
|
  T. Pearson, A. Y. Warren, D. A. Barrett, and R. N. Khan Detection of EETs and HETE-generating cytochrome P-450 enzymes and the effects of their metabolites on myometrial and vascular function Am J Physiol Endocrinol Metab, September 1, 2009; 297(3): E647 - E656. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. L. Pierce, J. D.K. Kresowik, K. G. Lamping, and S. K. England Overexpression of SK3 Channels Dampens Uterine Contractility to Prevent Preterm Labor in Mice Biol Reprod, June 1, 2008; 78(6): 1058 - 1063. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. P. Collis, S. Srivastava, W. A. Coetzee, and M. Artman beta2-Adrenergic receptor agonists stimulate L-type calcium current independent of PKA in newborn rabbit ventricular myocytes Am J Physiol Heart Circ Physiol, November 1, 2007; 293(5): H2826 - H2835. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Shmygol, K. Noble, and S. Wray Depletion of membrane cholesterol eliminates the Ca2+-activated component of outward potassium current and decreases membrane capacitance in rat uterine myocytes J. Physiol., June 1, 2007; 581(2): 445 - 456. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Smith Parturition N. Engl. J. Med., January 18, 2007; 356(3): 271 - 283. [Full Text] [PDF]
|
|
|
|
|
|
K. Noble, J. Zhang, and S. Wray Lipid rafts, the sarcoplasmic reticulum and uterine calcium signalling: an integrated approach J. Physiol., January 1, 2006; 570(1): 29 - 35. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Lu, A. Alioua, Y. Kumar, M. Eghbali, E. Stefani, and L. Toro MaxiK channel partners: physiological impact J. Physiol., January 1, 2006; 570(1): 65 - 72. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. M. Sanborn, C.-Y. Ku, S. Shlykov, and L. Babich Molecular Signaling Through G-Protein-Coupled Receptors and the Control of Intracellular Calcium in Myometrium Reproductive Sciences, October 1, 2005; 12(7): 479 - 487. [Abstract] [PDF]
|
|
|
|
|
|
M. Perusquia, E. Navarrete, J. Jasso-Kamel, and L. M. Montano Androgens Induce Relaxation of Contractile Activity in Pregnant Human Myometrium at Term: A Nongenomic Action on L-Type Calcium Channels Biol Reprod, August 1, 2005; 73(2): 214 - 221. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
