| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
MUSCLE CELL BIOLOGY AND CELL MOTILITY
1Department of Cellular and Integrative Physiology, Indiana University School of Medicine, Indianapolis, Indiana 46202; and 2Department of Cell and Developmental Biology, State University of New York Upstate Medical University, Syracuse, New York 13210
Submitted 17 January 2003 ; accepted in final form 17 October 2003
Cytoskeletal reorganization of the smooth muscle cell in response to contractile stimulation may be an important fundamental process in regulation of tension development. We used confocal microscopy to analyze the effects of cholinergic stimulation on localization of the cytoskeletal proteins vinculin, paxillin, talin and focal adhesion kinase (FAK) in freshly dissociated tracheal smooth muscle cells. All four proteins were localized at the membrane and throughout the cytoplasm of unstimulated cells, but their concentration at the membrane was greater in acetylcholine (ACh)-stimulated cells. Antisense oligonucleotides were introduced into tracheal smooth muscle tissues to deplete paxillin protein, which also inhibited contraction in response to ACh. In cells dissociated from paxillin-depleted muscle tissues, redistribution of vinculin to the membrane in response to ACh was prevented, but redistribution of FAK and talin was not inhibited. Muscle tissues were transfected with plasmids encoding a paxillin mutant containing a deletion of the LIM3 domain (paxillin LIM3 dl 444–494), the primary determinant for targeting paxillin to focal adhesions. Expression of paxillin LIM3 dl in muscle tissues also inhibited contractile force and prevented cellular redistribution of paxillin and vinculin to the membrane in response to ACh, but paxillin LIM3 dl did not inhibit increases in intracellular Ca2+ or myosin light chain phosphorylation. Our results demonstrate that recruitment of paxillin and vinculin to smooth muscle membrane is necessary for tension development and that recruitment of vinculin to the membrane is regulated by paxillin. Vinculin and paxillin may participate in regulating the formation of linkages between the cytoskeleton and integrin proteins that mediate tension transmission between the contractile apparatus and the extracellular matrix during smooth muscle contraction.
tissue transfection; plasmids; cytoskeleton; talin; immunofluorescence
This article has been cited by other articles:
|
|
 |
|
  S. Chen, R. Wang, Q.-F. Li, and D. D. Tang Abl knockout differentially affects p130 Crk-associated substrate, vinculin, and paxillin in blood vessels of mice Am J Physiol Heart Circ Physiol, August 1, 2009; 297(2): H533 - H539. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H.-L. Ding, J. W. Ryder, J. T. Stull, and K. E. Kamm Signaling Processes for Initiating Smooth Muscle Contraction upon Neural Stimulation J. Biol. Chem., June 5, 2009; 284(23): 15541 - 15548. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. D. Tang p130 Crk-Associated Substrate (CAS) in Vascular Smooth Muscle Journal of Cardiovascular Pharmacology and Therapeutics, June 1, 2009; 14(2): 89 - 98. [Abstract] [PDF]
|
|
|
|
 |
|
 Y. Wu, Y. Huang, B. P. Herring, and S. J. Gunst Integrin-linked kinase regulates smooth muscle differentiation marker gene expression in airway tissue Am J Physiol Lung Cell Mol Physiol, December 1, 2008; 295(6): L988 - L997. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. R. Kim, C. Gallant, P. C. Leavis, S. J. Gunst, and K. G. Morgan Cytoskeletal remodeling in differentiated vascular smooth muscle is actin isoform dependent and stimulus dependent Am J Physiol Cell Physiol, September 1, 2008; 295(3): C768 - C778. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. J. Gunst and W. Zhang Actin cytoskeletal dynamics in smooth muscle: a new paradigm for the regulation of smooth muscle contraction Am J Physiol Cell Physiol, September 1, 2008; 295(3): C576 - C587. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Srinivasan, S. Forman, R. A. Quinlan, J. Ohanian, and V. Ohanian Regulation of contractility by Hsp27 and Hic-5 in rat mesenteric small arteries Am J Physiol Heart Circ Physiol, February 1, 2008; 294(2): H961 - H969. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Zhang and S. J. Gunst Interactions of Airway Smooth Muscle Cells with Their Tissue Matrix: Implications for Contraction Proceedings of the ATS, January 1, 2008; 5(1): 32 - 39. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. T. Gerthoffer Migration of Airway Smooth Muscle Cells Proceedings of the ATS, January 1, 2008; 5(1): 97 - 105. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Anfinogenova, R. Wang, Q.-f. Li, A. M. Spinelli, and D. D. Tang Abl Silencing Inhibits CAS-Mediated Process and Constriction in Resistance Arteries Circ. Res., August 17, 2007; 101(4): 420 - 428. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Ali, L. Chin, P. D. Pare, and C. Y. Seow Mechanism of partial adaptation in airway smooth muscle after a step change in length J Appl Physiol, August 1, 2007; 103(2): 569 - 577. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. L. Corteling, S. E. Brett, H. Yin, X.-L. Zheng, M. P. Walsh, and D. G. Welsh The functional consequence of RhoA knockdown by RNA interference in rat cerebral arteries Am J Physiol Heart Circ Physiol, July 1, 2007; 293(1): H440 - H447. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. S. An, T. R. Bai, J. H. T. Bates, J. L. Black, R. H. Brown, V. Brusasco, P. Chitano, L. Deng, M. Dowell, D. H. Eidelman, et al. Airway smooth muscle dynamics: a common pathway of airway obstruction in asthma Eur. Respir. J., May 1, 2007; 29(5): 834 - 860. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Solway and C. G. Irvin Airway Smooth Muscle as a Target for Asthma Therapy N. Engl. J. Med., March 29, 2007; 356(13): 1367 - 1369. [Full Text] [PDF]
|
|
|
|
 |
|
 T. J. Eddinger, J. D. Schiebout, and D. R. Swartz Adherens junction-associated protein distribution differs in smooth muscle tissue and acutely isolated cells Am J Physiol Gastrointest Liver Physiol, February 1, 2007; 292(2): G684 - G697. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Wang, Q. Li, and D. D. Tang Role of vimentin in smooth muscle force development Am J Physiol Cell Physiol, September 1, 2006; 291(3): C483 - C489. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Zhang and S. J. Gunst Dynamic association between {alpha}-actinin and {beta}-integrin regulates contraction of canine tracheal smooth muscle J. Physiol., May 1, 2006; 572(3): 659 - 676. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. J. Eddinger, J. D. Schiebout, and D. R. Swartz Smooth muscle adherens junctions associated proteins are stable at the cell periphery during relaxation and activation Am J Physiol Cell Physiol, December 1, 2005; 289(6): C1379 - C1387. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Zhang, Y. Wu, L. Du, D. D. Tang, and S. J. Gunst Activation of the Arp2/3 complex by N-WASp is required for actin polymerization and contraction in smooth muscle Am J Physiol Cell Physiol, May 1, 2005; 288(5): C1145 - C1160. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. R. Kim, M. Hoque, and C.-M. Hai Cholinergic receptor-mediated differential cytoskeletal recruitment of actin- and integrin-binding proteins in intact airway smooth muscle Am J Physiol Cell Physiol, November 1, 2004; 287(5): C1375 - C1383. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. T. Sylvester The tone of pulmonary smooth muscle: ROK and Rho music? Am J Physiol Lung Cell Mol Physiol, October 1, 2004; 287(4): L624 - L630. [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
