HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: 285/1/C56    most recent 00377.2002v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (39) Citing Articles via Google Scholar Google Scholar Articles by Kubis, H.-P. Articles by Gros, G. Search for Related Content PubMed PubMed Citation Articles by Kubis, H.-P. Articles by Gros, G.

MUSCLE CELL BIOLOGY AND CELL MOTILITY

Ca²⁺ transients activate calcineurin/NFATc1 and initiate fast-to-slow transformation in a primary skeletal muscle culture

Zentrum Physiologie, Medizinische Hochschule Hannover, D-30625 Hannover, Germany

Submitted 20 August 2002 ; accepted in final form 11 February 2003

The calcineurin-mediated signal transduction via nuclear factor of activated T cells (NFATc1) is involved in upregulating slow myosin heavy chain (MHC) gene expression during fast-to-slow transformation of skeletal muscle cells. This study aims to investigate the Ca²⁺ signal necessary to activate the calcineurin-NFATc1 cascade in skeletal muscle. Electrostimulation of primary myocytes from rabbit for 24 h induced a distinct fast-to-slow transformation at the MHC mRNA level and a full activation of the calcineurin-NFATc1 pathway, although resting Ca²⁺ concentration ([Ca²⁺]_i) remained unaltered at 70 nM. During activation, the calcium transients of these myocytes reach a peak concentration of 500 nM. Although 70 nM [Ca²⁺]_i does not activate calcineurin-NFAT, we show by the use of Ca²⁺ ionophore that the system is fully activated when [Ca²⁺]_i is 150 nM in a sustained manner. We conclude that the calcineurin signal transduction pathway and the slow MHC gene in cultured skeletal muscle cells are activated by repetition of the rapid high-amplitude calcium transients that are associated with excitation-contraction coupling rather than by a sustained elevation of resting Ca²⁺ concentration.

muscle plasticity; NFATc1; resting calcium concentration

This article has been cited by other articles:

				J. T. Lanner, J. D. Bruton, Y. Assefaw-Redda, Z. Andronache, S.-J. Zhang, D. Severa, Z.-B. Zhang, W. Melzer, S.-L. Zhang, A. Katz, et al. Knockdown of TRPC3 with siRNA coupled to carbon nanotubes results in decreased insulin-mediated glucose uptake in adult skeletal muscle cells FASEB J, June 1, 2009; 23(6): 1728 - 1738. [Abstract] [Full Text] [PDF]

				W. A. LaFramboise, R. C. Jayaraman, K. L. Bombach, D. P. Ankrapp, J. M. Krill-Burger, C. M. Sciulli, P. Petrosko, and R. W. Wiseman Acute molecular response of mouse hindlimb muscles to chronic stimulation Am J Physiol Cell Physiol, January 1, 2009; 297(3): C556 - C570. [Abstract] [Full Text] [PDF]

				J. A. Drenning, V. A. Lira, C. G. Simmons, Q. A. Soltow, J. E. Sellman, and D. S. Criswell Nitric oxide facilitates NFAT-dependent transcription in mouse myotubes Am J Physiol Cell Physiol, April 1, 2008; 294(4): C1088 - C1095. [Abstract] [Full Text] [PDF]

				J. A. Valdes, E. Gaggero, J. Hidalgo, N. Leal, E. Jaimovich, and M. A. Carrasco NFAT activation by membrane potential follows a calcium pathway distinct from other activity-related transcription factors in skeletal muscle cells Am J Physiol Cell Physiol, March 1, 2008; 294(3): C715 - C725. [Abstract] [Full Text] [PDF]

				N. Dehne, U. Kerkweg, T. Otto, and J. Fandrey The HIF-1 response to simulated ischemia in mouse skeletal muscle cells neither enhances glycolysis nor prevents myotube cell death Am J Physiol Regulatory Integrative Comp Physiol, October 1, 2007; 293(4): R1693 - R1701. [Abstract] [Full Text] [PDF]

				X. Mu, L. D. Brown, Y. Liu, and M. F. Schneider Roles of the calcineurin and CaMK signaling pathways in fast-to-slow fiber type transformation of cultured adult mouse skeletal muscle fibers Physiol Genomics, August 20, 2007; 30(3): 300 - 312. [Abstract] [Full Text] [PDF]

				Y.-C. Huang, R. G. Dennis, and K. Baar Cultured slow vs. fast skeletal muscle cells differ in physiology and responsiveness to stimulation Am J Physiol Cell Physiol, July 1, 2006; 291(1): C11 - C17. [Abstract] [Full Text] [PDF]

				J. V. Chakkalakal, S. A. Michel, E. R. Chin, R. N. Michel, and B. J. Jasmin Targeted inhibition of Ca2+/calmodulin signaling exacerbates the dystrophic phenotype in mdx mouse muscle Hum. Mol. Genet., May 1, 2006; 15(9): 1423 - 1435. [Abstract] [Full Text] [PDF]

				S. L. McGee and M. Hargreaves Exercise and Myocyte Enhancer Factor 2 Regulation in Human Skeletal Muscle Diabetes, May 1, 2004; 53(5): 1208 - 1214. [Abstract] [Full Text] [PDF]

				P. G. Hogan, L. Chen, J. Nardone, and A. Rao Transcriptional regulation by calcium, calcineurin, and NFAT Genes & Dev., September 15, 2003; 17(18): 2205 - 2232. [Full Text] [PDF]