| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 Department of Pathology and Laboratory Medicine, 2 Jules Stein Eye Institute, 4 Department of Biological Chemistry, 5 Department of Neurobiology and Brain Research Institute, University of California, Los Angeles 90095; and 3 Retina-Vitreous Associates Medical Group, Los Angeles, California 90278
Na,K-ATPase regulates a variety of transport functions in epithelial cells. In cultures of human retinal pigment epithelial (RPE) cells, inhibition of Na,K-ATPase by ouabain and K+ depletion decreased transepithelial electrical resistance (TER) and increased permeability of tight junctions to mannitol and inulin. Electrophysiological studies demonstrated that the decrease in TER was due to an increase in paracellular shunt conductance. At the light microscopy level, this increased permeability was not accompanied by changes in the localization of the tight junction proteins ZO-1, occludin, and claudin-3. At the ultrastructural level, increased tight junction permeability correlated with a decrease in tight junction membrane contact points. Decreased tight junction membrane contact points and increased tight junction permeability were reversible in K+-repletion experiments. Confocal microscopy revealed that in control cells, Na,K-ATPase was localized at both apical and basolateral plasma membranes. K+ depletion resulted in a large reduction of apical Na,K-ATPase, and after K+ repletion the apical Na,K-ATPase recovered to control levels. These results suggest a functional link exists between Na,K-ATPase and tight junction function in human RPE cells.
actin stress fibers
This article has been cited by other articles:
|
|
 |
|
  T. Krupinski and G. J. Beitel Unexpected Roles of the Na-K-ATPase and Other Ion Transporters in Cell Junctions and Tubulogenesis Physiology, June 1, 2009; 24(3): 192 - 201. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. J. Clifford and J. H. Kaplan {beta}-Subunit overexpression alters the stoicheometry of assembled Na-K-ATPase subunits in MDCK cells Am J Physiol Renal Physiol, November 1, 2008; 295(5): F1314 - F1323. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. A. Newman, P. Yang, A. D. Pawlus, and K. I. Block Cardiac Glycosides as Novel Cancer Therapeutic Agents Mol. Interv., February 1, 2008; 8(1): 36 - 49. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. M. Mutlu, D. Machado-Aranda, J. E. Norton, A. Bellmeyer, D. Urich, R. Zhou, and D. A. Dean Electroporation-mediated Gene Transfer of the Na+,K+-ATPase Rescues Endotoxin-induced Lung Injury Am. J. Respir. Crit. Care Med., September 15, 2007; 176(6): 582 - 590. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Cibrian-Uhalte, A. Langenbacher, X. Shu, J.-N. Chen, and S. Abdelilah-Seyfried Involvement of zebrafish Na+,K+ ATPase in myocardial cell junction maintenance J. Cell Biol., January 16, 2007; 176(2): 223 - 230. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. A. Rajasekaran, S. P. Barwe, J. Gopal, S. Ryazantsev, E. E. Schneeberger, and A. K. Rajasekaran Na-K-ATPase regulates tight junction permeability through occludin phosphorylation in pancreatic epithelial cells Am J Physiol Gastrointest Liver Physiol, January 1, 2007; 292(1): G124 - G133. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. B. N. Lee, E. Huang, and H. J. Ward Tight junction biology and kidney dysfunction Am J Physiol Renal Physiol, January 1, 2006; 290(1): F20 - F34. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Silberberg, A. J. Charron, R. Bacallao, and A. Wandinger-Ness Mispolarization of desmosomal proteins and altered intercellular adhesion in autosomal dominant polycystic kidney disease Am J Physiol Renal Physiol, June 1, 2005; 288(6): F1153 - F1163. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Turowski, P. Adamson, J. Sathia, J. J. Zhang, S. E. Moss, G. W. Aylward, M. J. Hayes, N. Kanuga, and J. Greenwood Basement Membrane-Dependent Modification of Phenotype and Gene Expression in Human Retinal Pigment Epithelial ARPE-19 Cells Invest. Ophthalmol. Vis. Sci., August 1, 2004; 45(8): 2786 - 2794. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Turksen and T.-C. Troy Barriers built on claudins J. Cell Sci., May 15, 2004; 117(12): 2435 - 2447. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. K. Rajasekaran and S. A. Rajasekaran Role of Na-K-ATPase in the assembly of tight junctions Am J Physiol Renal Physiol, September 1, 2003; 285(3): F388 - F396. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
