| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Division of Molecular and Cellular Physiology, Department of Physiological Sciences, Biomedical Center, Lund University, SE-221 84 Lund, Sweden
Signaling mechanisms for
stretch-dependent growth and differentiation of vascular smooth muscle
were investigated in mechanically loaded rat portal veins in organ
culture. Stretch-dependent protein synthesis was found to depend on
endogenous release of angiotensin II. Autoradiography after
[35S]methionine incorporation revealed stretch-dependent
synthesis of several proteins, of which SM22 and actin were
particularly prominent. Inhibition of RhoA activity by cell-permeant C3
toxin increased tissue mechanical compliance and reduced
stretch-dependent extracellular signal-regulated kinase (ERK)1/2
activation, growth, and synthesis of actin and SM22, suggesting a role
of the actin cytoskeleton. In contrast, inhibition of Rho-associated
kinase by Y-27632 did not reduce ERK1/2 phosphorylation or actin and SM22 synthesis and did not affect tissue mechanical compliance but
still inhibited overall growth. The actin polymerization inhibitors latrunculin B and cytochalasin D both inhibited growth and caused increased tissue compliance. Whereas latrunculin B
concentration-dependently reduced actin and SM22 synthesis,
cytochalasin D did so at low (10
8 M) but not at high
(106 M) concentration. The results show that stretch
stabilizes the contractile smooth muscle phenotype. Stretch-dependent
differentiation marker expression requires an intact cytoskeleton for
stretch sensing, control of protein expression via the level of
unpolymerized G-actin, or both.
SM22; cytoskeleton; rat portal vein; RhoA; hypertension
This article has been cited by other articles:
|
|
 |
|
  Y.-M. Liou, M. Watanabe, M. Yumoto, and S. Ishiwata Regulatory mechanism of smooth muscle contraction studied with gelsolin-treated strips of taenia caeci in guinea pig Am J Physiol Cell Physiol, May 1, 2009; 296(5): C1024 - C1033. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. D. Luykenaar, R. A. El-Rahman, M. P. Walsh, and D. G. Welsh Rho-kinase-mediated suppression of KDR current in cerebral arteries requires an intact actin cytoskeleton Am J Physiol Heart Circ Physiol, April 1, 2009; 296(4): H917 - H926. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. R. Kim, C. Gallant, P. C. Leavis, S. J. Gunst, and K. G. Morgan Cytoskeletal remodeling in differentiated vascular smooth muscle is actin isoform dependent and stimulus dependent Am J Physiol Cell Physiol, September 1, 2008; 295(3): C768 - C778. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. J. Gunst and W. Zhang Actin cytoskeletal dynamics in smooth muscle: a new paradigm for the regulation of smooth muscle contraction Am J Physiol Cell Physiol, September 1, 2008; 295(3): C576 - C587. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Zhang, J. S. Helm, A. Senatore, J. D. Spafford, L. K. Kaczmarek, and E. A. Jonas PKC-Induced Intracellular Trafficking of CaV2 Precedes Its Rapid Recruitment to the Plasma Membrane J. Neurosci., March 5, 2008; 28(10): 2601 - 2612. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Albinsson, I. Nordstrom, K. Sward, and P. Hellstrand Differential dependence of stretch and shear stress signaling on caveolin-1 in the vascular wall Am J Physiol Cell Physiol, January 1, 2008; 294(1): C271 - C279. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. J. Sandoval, E. R. Injeti, W. T. Gerthoffer, and W. J. Pearce Postnatal maturation modulates relationships among cytosolic Ca2+, myosin light chain phosphorylation, and contractile tone in ovine cerebral arteries Am J Physiol Heart Circ Physiol, October 1, 2007; 293(4): H2183 - H2192. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Albinsson and P. Hellstrand Integration of signal pathways for stretch-dependent growth and differentiation in vascular smooth muscle Am J Physiol Cell Physiol, August 1, 2007; 293(2): C772 - C782. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. L. Corteling, S. E. Brett, H. Yin, X.-L. Zheng, M. P. Walsh, and D. G. Welsh The functional consequence of RhoA knockdown by RNA interference in rat cerebral arteries Am J Physiol Heart Circ Physiol, July 1, 2007; 293(1): H440 - H447. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. M. Nilsson, Z.-W. Sun, J. Nilsson, I. Nordstrom, Y.-W. Chen, J. D. Molkentin, D. Wide-Swensson, P. Hellstrand, M.-L. Lydrup, and M. F. Gomez Novel blocker of NFAT activation inhibits IL-6 production in human myometrial arteries and reduces vascular smooth muscle cell proliferation Am J Physiol Cell Physiol, March 1, 2007; 292(3): C1167 - C1178. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Loirand, P. Guerin, and P. Pacaud Rho Kinases in Cardiovascular Physiology and Pathophysiology Circ. Res., February 17, 2006; 98(3): 322 - 334. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. M. Nauli, J. M. Williams, W. T. Gerthoffer, and W. J. Pearce Chronic hypoxia modulates relations among calcium, myosin light chain phosphorylation, and force differently in fetal and adult ovine basilar arteries J Appl Physiol, July 1, 2005; 99(1): 120 - 127. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Makino, M. Glogauer, G. M. Bokoch, S. Chien, and G. W. Schmid-Schonbein Control of neutrophil pseudopods by fluid shear: role of Rho family GTPases Am J Physiol Cell Physiol, April 1, 2005; 288(4): C863 - C871. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Loirand, M. Rolli-Derkinderen, and P. Pacaud RhoA and resistance artery remodeling Am J Physiol Heart Circ Physiol, March 1, 2005; 288(3): H1051 - H1056. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Mayr, R. Siow, Y.-L. Chung, U. Mayr, J. R. Griffiths, and Q. Xu Proteomic and Metabolomic Analysis of Vascular Smooth Muscle Cells: Role of PKC{delta} Circ. Res., May 28, 2004; 94(10): e87 - e96. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Zhou, A. M. Hoggatt, and B. P. Herring Activation of the Smooth Muscle-specific Telokin Gene by Thyrotroph Embryonic Factor (TEF) J. Biol. Chem., April 16, 2004; 279(16): 15929 - 15937. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Barany and P. P. de Tombe Rapid exchange of actin-bound nucleotide in perfused rat heart Am J Physiol Heart Circ Physiol, April 1, 2004; 286(4): H1394 - H1401. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Johansson and K. Persson Phenotypic modulation of cultured bladder smooth muscle cells and the expression of inducible nitric oxide synthase Am J Physiol Regulatory Integrative Comp Physiol, April 1, 2004; 286(4): R642 - R648. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Zeidan, J. Broman, P. Hellstrand, and K. Sward Cholesterol Dependence of Vascular ERK1/2 Activation and Growth in Response to Stretch: Role of Endothelin-1 Arterioscler Thromb Vasc Biol, September 1, 2003; 23(9): 1528 - 1534. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
