Am J Physiol Cell Physiol Ad Instruments
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Cell Physiol 283: C1761-C1775, 2002. First published August 22, 2002; doi:10.1152/ajpcell.00278.2002
0363-6143/02 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
283/6/C1761    most recent
00278.2002v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (9)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Tsika, R. W.
Right arrow Articles by Tsika, G. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Tsika, R. W.
Right arrow Articles by Tsika, G. L.
Vol. 283, Issue 6, C1761-C1775, December 2002

Divergence in species and regulatory role of beta -myosin heavy chain proximal promoter muscle-CAT elements

Richard W. Tsika1,2,3, John McCarthy2, Natalia Karasseva1, Yangsi Ou2, and Gretchen L. Tsika2

1 Department of Biochemistry, School of Medicine, 2 Department of Biomedical Sciences, School of Veterinary Medicine, and 3 Dalton Cardiovascular Research Center, University of Missouri-Columbia, Columbia, Missouri 65211

We examined the functional role of distinct muscle-CAT (MCAT) elements during non-weight-bearing (NWB) regulation of a wild-type 293-base pair beta -myosin heavy chain (beta MyHC) transgene. Electrophoretic mobility shift assays (EMSA) revealed decreased NTEF-1, poly(ADP-ribose) polymerase, and Max binding at the human distal MCAT element when using NWB soleus vs. control soleus nuclear extract. Compared with the wild-type transgene, expression assays revealed that distal MCAT element mutation decreased basal transgene expression, which was decreased further in response to NWB. EMSA analysis of the human proximal MCAT (pMCAT) element revealed low levels of NTEF-1 binding that did not differ between control and NWB extract, whereas the rat pMCAT element displayed robust NTEF-1 binding that decreased when using NWB soleus extracts. Differences in binding between human and rat pMCAT elements were consistent whether using rat or mouse nuclear extract or in vitro synthesized human TEF-1 proteins. Our results provide the first evidence that 1) different binding properties and likely regulatory functions are served by the human and rat pMCAT elements, and 2) previously unrecognized beta MyHC proximal promoter elements contribute to NWB regulation.

skeletal muscle hypertrophy; skeletal muscle atrophy; fiber-type transitions; chloramphenicol acetyltransferase


This article has been cited by other articles:


Home page
 Am. J. Physiol. Cell Physiol.Home page
R. A. Shanely, K. A. Zwetsloot, T. E. Childs, S. J. Lees, R. W. Tsika, and F. W. Booth
IGF-I activates the mouse type IIb myosin heavy chain gene
Am J Physiol Cell Physiol, October 1, 2009; 297(4): C1019 - C1027.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
J. Ji, G. L. Tsika, H. Rindt, K. L. Schreiber, J. J. McCarthy, R. J. Kelm Jr., and R. Tsika
Pur{alpha} and Pur{beta} Collaborate with Sp3 To Negatively Regulate {beta}-Myosin Heavy Chain Gene Expression during Skeletal Muscle Inactivity
Mol. Cell. Biol., February 15, 2007; 27(4): 1531 - 1543.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
J. M. Giger, F. Haddad, A. X. Qin, M. Zeng, and K. M. Baldwin
Effect of unloading on type I myosin heavy chain gene regulation in rat soleus muscle
J Appl Physiol, April 1, 2005; 98(4): 1185 - 1194.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
G. Tsika, J. Ji, and R. Tsika
Sp3 Proteins Negatively Regulate {beta} Myosin Heavy Chain Gene Expression during Skeletal Muscle Inactivity
Mol. Cell. Biol., December 15, 2004; 24(24): 10777 - 10791.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
H.-H. Chen, S. J. Mullett, and A. F. R. Stewart
Vgl-4, a Novel Member of the Vestigial-like Family of Transcription Cofactors, Regulates {alpha}1-Adrenergic Activation of Gene Expression in Cardiac Myocytes
J. Biol. Chem., July 16, 2004; 279(29): 30800 - 30806.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
N. Karasseva, G. Tsika, J. Ji, A. Zhang, X. Mao, and R. Tsika
Transcription Enhancer Factor 1 Binds Multiple Muscle MEF2 and A/T-Rich Elements during Fast-to-Slow Skeletal Muscle Fiber Type Transitions
Mol. Cell. Biol., August 1, 2003; 23(15): 5143 - 5164.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online