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Am J Physiol Cell Physiol 283: C1675-C1686, 2002. First published August 22, 2002; doi:10.1152/ajpcell.00092.2002
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Vol. 283, Issue 6, C1675-C1686, December 2002

Endothelial cells maintain a reduced redox environment even as mitochondrial function declines

Ricarda Carlisle, Carol Ann Rhoads, Tak Yee Aw, and Lynn Harrison

Department of Molecular and Cellular Physiology, Louisiana Health Sciences Center, Shreveport, Louisiana

Human umbilical vein endothelial cells (HUVECs) are an endothelial model of replicative senescence. Oxidative stress, possibly due to dysfunctional mitochondria, is believed to play a key role in replicative senescence and atherosclerosis, an age-related vascular disease. In this study, we determined the effect of cell division on genomic instability, mitochondrial function, and redox status in HUVECs that were able to replicate for ~60 cumulative population doublings (CPD). After 20 CPD, the nuclear genome deteriorated and the protein content of the cell population increased. This indicated an increase in cell size, which was accompanied by an increase in oxygen consumption, ATP production, and mitochondrial genome copy number and ~10% increase in mitochondrial mass. The antioxidant capacity increased, as seen by an increase in reduced glutathione, glutathione peroxidase, GSSG reductase, and glucose-6-phosphate dehydrogenase. However, by CPD 52, the latter two enzymes decreased, as well as the ratio of mitochondrial-to-nuclear genome copies, the mitochondrial mass, and the oxygen consumption per milligram of protein. Our results signify that HUVECs maintain a highly reducing (GSH) environment as they replicate despite genomic instability and loss of mitochondrial function.

replicative senescence; glutathione; cell size changes; genomic instability; human umbilical vein endothelial cells


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