Am J Physiol Cell Physiol AJP: Regulatory, Integrative and Comparative Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Cell Physiol 283: C1414-C1421, 2002. First published August 14, 2002; doi:10.1152/ajpcell.00135.2002
0363-6143/02 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
283/5/C1414    most recent
00135.2002v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (12)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Dubois, S. G.
Right arrow Articles by Lajeunesse, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Dubois, S. G.
Right arrow Articles by Lajeunesse, D.
Vol. 283, Issue 5, C1414-C1421, November 2002

Role of abnormal neutral endopeptidase-like activities in Hyp mouse bone cells in renal phosphate transport

Stéphanie G. Dubois1, Andréa Frota Ruchon2, Aline Delalandre1, Guy Boileau2, and Daniel Lajeunesse1

1 Unité de recherche en Arthrose, Centre de Recherche du Centre Hospitalier de l'Université de Montréal, Hôpital Notre-Dame, Montreal H2L 4M1; and 2 Département de Biochimie, Université de Montréal, Montreal, Quebec, Canada H3C 3J7

We investigated whether the absence of Phex (phosphate-regulating gene with homologies to endopeptidases on the X chromosome) in the Hyp mouse affects the expression and activity of neprilysin (NEP) and of endothelin-converting enzyme-like endopeptidase (ECEL1/DINE) in bone marrow stromal cells (BMSC) and osteoblasts (Ob). Total NEP-like activity was higher in Ob than in BMSC regardless of genotype, and Hyp cells showed higher activities than normal. Conditioned media (CM) from Hyp BMSC and Ob inhibited inorganic phosphate (Pi) uptake by mouse proximal tubule cells, and incubating Hyp Ob with phosphoramidon prevented the production of the inhibitor of renal Pi uptake. A linear relationship was observed between the NEP-like activity of Hyp and normal cells and the inhibition of Pi uptake. NEP and ECEL1/DINE mRNA levels were higher in Hyp cells than in normal cells, and in situ hybridization of ECEL1/DINE confirmed higher levels of expression in the Hyp mouse than in normal cells. In conclusion, we observed a correlation between the inhibition of Pi uptake by CM from Hyp cells and elevated NEP-like activities.

X-linked hypophosphatemia; Phex; putative inhibitor of renal phosphate uptake


This article has been cited by other articles:


Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
N. Matsumoto, O. D. Jo, R. N. J. Shih, E. J. Brochmann, S. S. Murray, V. Hong, J. Yanagawa, and N. Yanagawa
Increased cathepsin D release by Hyp mouse osteoblast cells
Am J Physiol Endocrinol Metab, July 1, 2005; 289(1): E123 - E132.
[Abstract] [Full Text] [PDF]

Home page
 CROBMHome page
P. S.N. Rowe
THE WRICKKENED PATHWAYS OF FGF23, MEPE AND PHEX
Critical Reviews in Oral Biology & Medicine, September 1, 2004; 15(5): 264 - 281.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
A. J. Brewer, L. Canaff, G. N. Hendy, and H. S. Tenenhouse
Differential regulation of PHEX expression in bone and parathyroid gland by chronic renal insufficiency and 1,25-dihydroxyvitamin D3
Am J Physiol Renal Physiol, April 1, 2004; 286(4): F739 - F748.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
L. D. Quarles
FGF23, PHEX, and MEPE regulation of phosphate homeostasis and skeletal mineralization
Am J Physiol Endocrinol Metab, July 1, 2003; 285(1): E1 - E9.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online