Am J Physiol Cell Physiol Email Content Delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Cell Physiol 282: C1076-C1086, 2002. First published December 12, 2001; doi:10.1152/ajpcell.00210.2001
0363-6143/02 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
282/5/C1076    most recent
00210.2001v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (26)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Morimoto, N.
Right arrow Articles by Brownell, W. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Morimoto, N.
Right arrow Articles by Brownell, W. E.
Vol. 282, Issue 5, C1076-C1086, May 2002

Excess plasma membrane and effects of ionic amphipaths on mechanics of outer hair cell lateral wall

Noriko Morimoto, Robert M. Raphael, Anders Nygren, and William E. Brownell

Department of Otorhinolaryngology and Communicative Science, Baylor College of Medicine, Houston, Texas 77030

The interaction between the outer hair cell (OHC) lateral wall plasma membrane and the underlying cortical lattice was examined by a morphometric analysis of cell images during cell deformation. Vesiculation of the plasma membrane was produced by micropipette aspiration in control cells and cells exposed to ionic amphipaths that alter membrane mechanics. An increase of total cell and vesicle surface area suggests that the plasma membrane possesses a membrane reservoir. Chlorpromazine (CPZ) decreased the pressure required for vesiculation, whereas salicylate (Sal) had no effect. The time required for vesiculation was decreased by CPZ, indicating that CPZ decreases the energy barrier required for vesiculation. An increase in total volume is observed during micropipette aspiration. A deformation-induced increase in hydraulic conductivity is also seen in response to micropipette-applied fluid jet deformation of the lateral wall. Application of CPZ and/or Sal decreased this strain-induced hydraulic conductivity. The impact of ionic amphipaths on OHC plasma membrane and lateral wall mechanics may contribute to their effects on OHC electromotility and hearing.

membrane reservoir; electromotility; membrane bending; hearing; hydraulic conductivity


This article has been cited by other articles:


Home page
 Physiol. Rev.Home page
J. Ashmore
Cochlear Outer Hair Cell Motility
Physiol Rev, January 1, 2008; 88(1): 173 - 210.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
S. A. Ermilov, D. R. Murdock, D. El-Daye, W. E. Brownell, and B. Anvari
Effects of Salicylate on Plasma Membrane Mechanics
J Neurophysiol, September 1, 2005; 94(3): 2105 - 2110.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
V. Rybalchenko and J. Santos-Sacchi
Cl- flux through a non-selective, stretch-sensitive conductance influences the outer hair cell motor of the guinea-pig
J. Physiol., March 15, 2003; 547(3): 873 - 891.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online