| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Departments of 1 Surgery, 4 Physiology, and 5 Pathology, University of Maryland School of Medicine and 2 Baltimore Veterans Affairs Medical Center, Baltimore, Maryland 21201; and 3 Department of Medicine, School of Medicine, University of California, San Diego, California 92103
Early mucosal restitution occurs by epithelial cell migration to reseal superficial wounds after injury. Differentiated intestinal epithelial cells induced by forced expression of the Cdx2 gene migrate over the wounded edge much faster than undifferentiated parental cells in an in vitro model. This study determined whether these differentiated intestinal epithelial cells exhibit increased migration by altering voltage-gated K+ (Kv) channel expression and cytosolic free Ca2+ concentration ([Ca2+]cyt). Stable Cdx2-transfected IEC-6 cells (IEC-Cdx2L1) with highly differentiated phenotype expressed higher basal levels of Kv1.1 and Kv1.5 mRNAs and proteins than parental IEC-6 cells. Neither IEC-Cdx2L1 cells nor parental IEC-6 cells expressed voltage-dependent Ca2+ channels. The increased expression of Kv channels in differentiated IEC-Cdx2L1 cells was associated with an increase in whole cell K+ currents, membrane hyperpolarization, and a rise in [Ca2+]cyt. The migration rates in differentiated IEC-Cdx2L1 cells were about four times those of parental IEC-6 cells. Inhibition of Kv channel expression by polyamine depletion decreased [Ca2+]cyt, reduced myosin stress fibers, and inhibited cell migration. Elevation of [Ca2+]cyt by ionomycin promoted myosin II stress fiber formation and increased cell migration. These results suggest that increased migration of differentiated intestinal epithelial cells is mediated, at least partially, by increasing Kv channel activity and Ca2+ influx during restitution.
voltage-gated potassium channels; intracellular calcium; membrane potential; restitution; Cdx2 gene; differentiation; polyamines
This article has been cited by other articles:
|
|
 |
|
  N. T. N. Trinh, A. Prive, E. Maille, J. Noel, and E. Brochiero EGF and K+ channel activity control normal and cystic fibrosis bronchial epithelia repair Am J Physiol Lung Cell Mol Physiol, November 1, 2008; 295(5): L866 - L880. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Chen, L. Xiao, J. N. Rao, T. Zou, L. Liu, E. Bellavance, M. Gorospe, and J.-Y. Wang JunD Represses Transcription and Translation of the Tight Junction Protein Zona Occludens-1 Modulating Intestinal Epithelial Barrier Function Mol. Biol. Cell, September 1, 2008; 19(9): 3701 - 3712. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Schwab, P. Hanley, A. Fabian, and C. Stock Potassium Channels Keep Mobile Cells on the Go Physiology, August 1, 2008; 23(4): 212 - 220. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Heitzmann and R. Warth Physiology and Pathophysiology of Potassium Channels in Gastrointestinal Epithelia Physiol Rev, July 1, 2008; 88(3): 1119 - 1182. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. N. Raveendran, K. Silver, L. C. Freeman, D. Narvaez, K. Weng, S. Ganta, and J. D. Lillich Drug-Induced Alterations to Gene and Protein Expression in Intestinal Epithelial Cell 6 Cells Suggest a Role for Calpains in the Gastrointestinal Toxicity of Nonsteroidal Anti-Inflammatory Agents J. Pharmacol. Exp. Ther., May 1, 2008; 325(2): 389 - 399. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. S. Marasa, L. Xiao, J. N. Rao, T. Zou, L. Liu, J. Wang, E. Bellavance, D. J. Turner, and J.-Y. Wang Induced TRPC1 expression increases protein phosphatase 2A sensitizing intestinal epithelial cells to apoptosis through inhibition of NF-{kappa}B activation Am J Physiol Cell Physiol, May 1, 2008; 294(5): C1277 - C1287. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. T. N. Trinh, A. Prive, L. Kheir, J.-C. Bourret, T. Hijazi, M. G. Amraei, J. Noel, and E. Brochiero Involvement of KATP and KvLQT1 K+ channels in EGF-stimulated alveolar epithelial cell repair processes Am J Physiol Lung Cell Mol Physiol, October 1, 2007; 293(4): L870 - L882. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. V. Remillard, D. D. Tigno, O. Platoshyn, E. D. Burg, E. E. Brevnova, D. Conger, A. Nicholson, B. K. Rana, R. N. Channick, L. J. Rubin, et al. Function of Kv1.5 channels and genetic variations of KCNA5 in patients with idiopathic pulmonary arterial hypertension Am J Physiol Cell Physiol, May 1, 2007; 292(5): C1837 - C1853. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. N. Rao, L. Liu, T. Zou, B. S. Marasa, D. Boneva, S. R. Wang, D. L. Malone, D. J. Turner, and J.-Y. Wang Polyamines are required for phospholipase C-{gamma}1 expression promoting intestinal epithelial restitution after wounding Am J Physiol Gastrointest Liver Physiol, January 1, 2007; 292(1): G335 - G343. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. N. Rao, O. Platoshyn, V. A. Golovina, L. Liu, T. Zou, B. S. Marasa, D. J. Turner, J. X.-J. Yuan, and J.-Y. Wang TRPC1 functions as a store-operated Ca2+ channel in intestinal epithelial cells and regulates early mucosal restitution after wounding Am J Physiol Gastrointest Liver Physiol, April 1, 2006; 290(4): G782 - G792. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Grishin, H. Ford, J. Wang, H. Li, V. Salvador-Recatala, E. S. Levitan, and E. Zaks-Makhina Attenuation of apoptosis in enterocytes by blockade of potassium channels Am J Physiol Gastrointest Liver Physiol, November 1, 2005; 289(5): G815 - G821. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Guo, J. N. Rao, L. Liu, T. Zou, K. M. Keledjian, D. Boneva, B. S. Marasa, and J.-Y. Wang Polyamines are necessary for synthesis and stability of occludin protein in intestinal epithelial cells Am J Physiol Gastrointest Liver Physiol, June 1, 2005; 288(6): G1159 - G1169. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Guo, J. N. Rao, L. Liu, T.-T. Zou, D. J. Turner, B. L. Bass, and J.-Y. Wang Regulation of adherens junctions and epithelial paracellular permeability: a novel function for polyamines Am J Physiol Cell Physiol, November 1, 2003; 285(5): C1174 - C1187. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Y. Lee, P. J. Maniak, D. H. Ingbar, and S. M. O'Grady Adult alveolar epithelial cells express multiple subtypes of voltage-gated K+ channels that are located in apical membrane Am J Physiol Cell Physiol, June 1, 2003; 284(6): C1614 - C1624. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. N. Rao, X. Guo, L. Liu, T. Zou, K. S. Murthy, J. X.-J. Yuan, and J.-Y. Wang Polyamines regulate Rho-kinase and myosin phosphorylation during intestinal epithelial restitution Am J Physiol Cell Physiol, April 1, 2003; 284(4): C848 - C859. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Guo, J. N. Rao, L. Liu, M. Rizvi, D. J. Turner, and J.-Y. Wang Polyamines regulate beta -catenin tyrosine phosphorylation via Ca2+ during intestinal epithelial cell migration Am J Physiol Cell Physiol, September 1, 2002; 283(3): C722 - C734. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
