| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1 Department of Physiology, Nagoya City University Medical School, Nagoya 467-8601; and 2 Department of Pharmacology, Juntendo University School of Medicine, Tokyo 113-8421, Japan
The type 1 ryanodine
receptor (RyR1) from rabbit skeletal muscle displayed two distinct
degrees of response to cytoplasmic Ca2+ [high- and
low-open probability (Po) channels]. Here, we
examined the effects of adenine nucleotides and caffeine on these
channels and their modulations by sulfhydryl reagents.
High-Po channels showed biphasic
Ca2+ dependence and were activated by adenine nucleotides
and caffeine. Unexpectedly, low-Po channels did
not respond to either modulator. The addition of a reducing reagent,
dithiothreitol, to the cis side converted the
high-Po channel to a state similar to that of
the low-Po channel. Treatment with
p-chloromercuriphenylsulfonic acid (pCMPS) transformed
low-Po channels to a
high-Po channel-like state with stimulation by
,
-methylene-ATP and caffeine. In experiments under redox control
using glutathione buffers, shift of the cis potential toward
the oxidative state activated the low-Po
channel, similar to that of the high-Po or the
pCMPS-treated channel, whereas reductive changes inactivated the
high-Po channel. Changes in trans
redox potential, in contrast, did not affect channel activity of
either channel. In all experiments, channels with higher
Po were stimulated to a great extent by
modulators, but ones with lower Po were
unresponsive. These results suggest that redox states of critical
sulfhydryls located on the cytoplasmic side of the RyR1 may alter both
gating properties of the channel and responsiveness to channel modulators.
calcium-induced calcium release; adenine nucleotide; caffeine; sulfhydryl reagents; redox potential
This article has been cited by other articles:
|
|
 |
|
  B. T. Corona, C. Rouviere, S. L. Hamilton, and C. P. Ingalls Eccentric contractions do not induce rhabdomyolysis in malignant hyperthermia susceptible mice J Appl Physiol, November 1, 2008; 105(5): 1542 - 1553. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Oba, Y. Maeno, M. Nagao, N. Sakuma, and T. Murayama Cellular redox state protects acetaldehyde-induced alteration in cardiomyocyte function by modifying Ca2+ release from sarcoplasmic reticulum Am J Physiol Heart Circ Physiol, January 1, 2008; 294(1): H121 - H133. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Hidalgo Cross talk between Ca2+ and redox signalling cascades in muscle and neurons through the combined activation of ryanodine receptors/Ca2+ release channels Phil Trans R Soc B, December 29, 2005; 360(1464): 2237 - 2246. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Murayama, T. Oba, S. Kobayashi, N. Ikemoto, and Y. Ogawa Postulated role of interdomain interactions within the type 1 ryanodine receptor in the low gain of Ca2+-induced Ca2+ release activity of mammalian skeletal muscle sarcoplasmic reticulum Am J Physiol Cell Physiol, June 1, 2005; 288(6): C1222 - C1230. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Oba and Y. Maeno Acetaldehyde alters Ca2+-release channel gating and muscle contraction in a dose-dependent manner Am J Physiol Cell Physiol, May 1, 2004; 286(5): C1188 - C1194. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Bull, J. J. Marengo, J. P. Finkelstein, M. I. Behrens, and O. Alvarez SH oxidation coordinates subunits of rat brain ryanodine receptor channels activated by calcium and ATP Am J Physiol Cell Physiol, July 1, 2003; 285(1): C119 - C128. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Lloyd-Evans, D. Pelled, C. Riebeling, J. Bodennec, A. de-Morgan, H. Waller, R. Schiffmann, and A. H. Futerman Glucosylceramide and Glucosylsphingosine Modulate Calcium Mobilization from Brain Microsomes via Different Mechanisms J. Biol. Chem., June 20, 2003; 278(26): 23594 - 23599. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. D. Thorne and R. J. Paul Effects of organ culture on arterial gene expression and hypoxic relaxation: role of the ryanodine receptor Am J Physiol Cell Physiol, April 1, 2003; 284(4): C999 - C1005. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Oba, C. Kurono, R. Nakajima, T. Takaishi, K. Ishida, G. A. Fuller, W. Klomkleaw, and M. Yamaguchi H2O2 activates ryanodine receptor but has little effect on recovery of releasable Ca2+ content after fatigue J Appl Physiol, December 1, 2002; 93(6): 1999 - 2008. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Fill and J. A. Copello Ryanodine Receptor Calcium Release Channels Physiol Rev, October 1, 2002; 82(4): 893 - 922. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
