Am J Physiol Cell Physiol AJP: Gastrointestinal and Liver Physiology
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Am J Physiol Cell Physiol (October 3, 2001). doi:10.1152/ajpcell.01396.2000
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Articles in PresS, published online ahead of print October 3, 2001
Am J Physiol Cell Physiol, 10.1152/ajpcell.01396.2000
Submitted on December 22, 2000
Accepted on September 25, 2001

Growth factors regulation of uncoupling protein-1 mRNA expression in brown adipocytes

Bibian Garcia1 and Maria-Jesus Obregon1*

1 Endocrinologia Molecular, Instituto de Investigaciones Biomedicas (CSIC), 28029 Madrid, Spain

* To whom correspondence should be addressed. E-mail: mjobregon{at}iib.uam.es.

To study the effect of the mitogens epidermal growth factor (EGF), acidic and basic fibroblast growth factors (aFGF and bFGF) and vasopressin on brown adipocyte differentiation, we analyzed the expression of uncoupling protein (UCP-1) mRNA. Quiescent brown preadipocytes express high levels of UCP-1 mRNA in response to triiodothyronine (T3) and norepinephrine (NE). The addition of serum or the mitogenic conditions aFGF+vasopressin+NE, or EGF+vasopressin+NE, decreases UCP-1 mRNA. A second addition of mitogens further decreases UCP-1 mRNA. Treatment with aFGF or bFGF alone, increases UCP-1 mRNA, while the addition of EGF or vasopressin dramatically reduces UCP-1 mRNA levels. The continuous presence of T3 increases UCP-1 mRNA levels in cells treated with EGF, aFGF or bFGF. The effect of T3 on the stimulation of DNA synthesis was also tested. T3 inhibits the mitogenic activity of aFGF and bFGF. In conclusion, mitogens like aFGF or bFGF allow brown adipocyte differentiation, while EGF and vasopressin inhibit the differentiation process. T3 behaves as an important hormone that regulates both brown adipocyte proliferation and differentiation.




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