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Am J Physiol Cell Physiol (February 6, 2002). doi:10.1152/ajpcell.00612.2001
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Articles in PresS, published online ahead of print February 6, 2002
Am J Physiol Cell Physiol, 10.1152/ajpcell.00612.2001
Submitted on December 26, 2001
Accepted on January 31, 2002

In vivo microdialysis for the determination of nasal liquid ion composition

Barbara R Grubb1*, James L Chadburn1, and Richard C Boucher1

1 CF/Pulmonary Research and Treatment Center, The University of North Carolina at Chapel Hill, Chapel Hill, NC, USA

* To whom correspondence should be addressed. E-mail: bgrubb{at}med.unc.edu.

Airway surface liquid (ASL) contains substances important in mucociliary clearance and airways defense. Little is known about substance concentrations in ASL due to its small volume and sampling difficulties. We used in vivo microdialysis (IVMD) to sample liquid lining the nasal cavity without net volume removal and incorporated into IMVD a potential difference (PD) electrode to assess airway integrity. The cystic fibrosis (CF) mouse nasal epithelia exhibit ion transport defects identical to those in CF human airways, and thus are a good model for CF disease. We determined that nasal liquid [Na+] [107 ± 4 mM normal (Nl); 111 ± 9 mM CF] and [Cl-] (120 ± 6 mM Nl; 122 ± 4 mM CF) did not differ between genotypes. The nasal liquid [K+] (8.7 ± 0.4 mM) was significantly less in NL than in CF mice (16.6 ± 4 mM). IVMD accurately samples nasal liquid for ionic composition. The ionic composition of nasal liquid in the Nl and CF mice is similar.




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