| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Articles in PresS, published online ahead of print July 3, 2002
Am J Physiol Cell Physiol, 10.1152/ajpcell.00607.2001
Submitted on December 20, 2001
Accepted on June 25, 2002
1 Program in Development and Fetal Health, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada
2 Toronto General Research Institute, Toronto, Ontario, Canada; Departments of Obstetrics and Gynecology and Physiology, University of Toronto, Toronto, Ontario, Canada
3 Program in Development and Fetal Health, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada; Departments of Obstetrics and Gynecology and Physiology, University of Toronto, Toronto, Ontario, Canada
* To whom correspondence should be addressed. E-mail: Lye{at}mshri.on.ca.
Evidence indicates that stretch of the uterus imposed by the growing fetus contributes to the onset of labor. Previously we have shown that mechanically stretching rat myometrial smooth muscle cells (SMCs) induces c-fos expression. To investigate this stretch-induced signaling we examined the involvement of the mitogen-activated protein kinase (MAPK) family. We show that stretching rat myometrial SMCs induces a rapid and transient phosphorylation (activation) of MAPKs: extracellular signal-regulated protein kinase (ERK), c-Jun N-terminal kinase (JNK), and p38. Use of selective inhibitors for the ERK pathway (PD98059 and U0126), p38 (SB203580) and JNK pathway (curcumin) demonstrated that activation of all three MAPK signaling pathways was necessary for optimal stretch-induced c-fos expression. We also demonstrate that upstream tyrosine kinase activity is involved in the mechanotransduction pathway leading to stretch-induced MAPK activation and c-fos mRNA expression. To further examine the role of MAPKs in vivo we used a unilaterally pregnant rat model. MAPKs (ERK and p38) are expressed in the pregnant rat myometrium with maximal ERK and p38 phosphorylation occurring in the 24 hours immediately preceding labour. Importantly, the rise in MAPK phosphorylation was confined to the gravid horn and absent in the empty uterine horn, suggesting that mechanical strain imposed by the growing fetus controls MAPK activation in the myometrium. Collectively this data indicate that mechanical stretch modulates MAPK activity in the myometrium leading to c-fos expression.
This article has been cited by other articles:
|
|
 |
|
  O. Shynlova, M. Chow, and S. J. Lye Expression and Organization of Basement Membranes and Focal Adhesion Proteins in Pregnant Myometrium is Regulated by Uterine Stretch Reproductive Sciences, October 1, 2009; 16(10): 960 - 969. [Abstract] [PDF]
|
|
|
|
|
|
M. Phillippe, L. M. Sweet, D. F. Bradley, and D. Engle Role of Nonreceptor Protein Tyrosine Kinases During Phospholipase C-{gamma}1-related Uterine Contractions in the Rat Reproductive Sciences, March 1, 2009; 16(3): 265 - 273. [Abstract] [PDF]
|
|
|
|
 |
|
 T. Peng, T. Zhang, X. Lu, and Q. Feng JNK1/c-fos inhibits cardiomyocyte TNF-{alpha} expression via a negative crosstalk with ERK and p38 MAPK in endotoxaemia Cardiovasc Res, March 1, 2009; 81(4): 733 - 741. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Garcia-Verdugo, Z. Tanfin, E. Dallot, M.-J. Leroy, and M. Breuiller-Fouche Surfactant Protein A Signaling Pathways in Human Uterine Smooth Muscle Cells Biol Reprod, August 1, 2008; 79(2): 348 - 355. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Shynlova, S. J. Williams, H. Draper, B. G White, D. J MacPhee, and S. J Lye Uterine Stretch Regulates Temporal and Spatial Expression of Fibronectin Protein and Its Alpha 5 Integrin Receptor in Myometrium of Unilaterally Pregnant Rats Biol Reprod, November 1, 2007; 77(5): 880 - 888. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Garcia-Verdugo, D. Leiber, P. Robin, E. Billon-Denis, R. Chaby, and Z. Tanfin Direct Interaction of Surfactant Protein A with Myometrial Binding Sites: Signaling and Modulation by Bacterial Lipopolysaccharide Biol Reprod, April 1, 2007; 76(4): 681 - 691. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Dalrymple, K. Mahn, L. Poston, E. Songu-Mize, and R.M. Tribe Mechanical stretch regulates TRPC expression and calcium entry in human myometrial smooth muscle cells Mol. Hum. Reprod., March 1, 2007; 13(3): 171 - 179*. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Smith Parturition N. Engl. J. Med., January 18, 2007; 356(3): 271 - 283. [Full Text] [PDF]
|
|
|
|
 |
|
 R. M. McAdams, S. B. Mustafa, J. S. Shenberger, P. S. Dixon, B. M. Henson, and R. J. DiGeronimo Cyclic stretch attenuates effects of hyperoxia on cell proliferation and viability in human alveolar epithelial cells Am J Physiol Lung Cell Mol Physiol, August 1, 2006; 291(2): L166 - L174. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. A. Radi and N. K. Khan Comparative Expression and Distribution of c-fos, Estrogen Receptor{alpha} (ER{alpha}), and p38{alpha} in the Uterus of Rats, Monkeys, and Humans Toxicol Pathol, June 1, 2006; 34(4): 327 - 335. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S.J. Williams, B.G. White, and D.J. MacPhee Expression of {alpha}5 Integrin (Itga5) Is Elevated in the Rat Myometrium During Late Pregnancy and Labor: Implications for Development of a Mechanical Syncytium Biol Reprod, May 1, 2005; 72(5): 1114 - 1124. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. A. Otun, M. W.J. MacDougall, J. Bailey, G. N. Europe-Finner, and S. C. Robson Spatial and Temporal Expression of the Myometrial Mitogen-Activated Protein Kinases p38 and ERK1/2 in the Human Uterus During Pregnancy and Labor Reproductive Sciences, April 1, 2005; 12(3): 185 - 190. [Abstract] [PDF]
|
|
|
|
|
|
J.A.Z. Loudon, S.R. Sooranna, P.R. Bennett, and M.R. Johnson Mechanical stretch of human uterine smooth muscle cells increases IL-8 mRNA expression and peptide synthesis Mol. Hum. Reprod., December 1, 2004; 10(12): 895 - 899. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. A. Mitchell, O. Shynlova, B. L. Langille, and S. J. Lye Mechanical stretch and progesterone differentially regulate activator protein-1 transcription factors in primary rat myometrial smooth muscle cells Am J Physiol Endocrinol Metab, September 1, 2004; 287(3): E439 - E445. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Tanabe, M. Koga, M. Saito, Y. Matsunaga, and K. Nakayama Inhibition of adipocyte differentiation by mechanical stretching through ERK-mediated downregulation of PPAR{gamma}2 J. Cell Sci., July 15, 2004; 117(16): 3605 - 3614. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Shynlova, J. A. Mitchell, A. Tsampalieros, B. L. Langille, and S. J. Lye Progesterone and Gravidity Differentially Regulate Expression of Extracellular Matrix Components in the Pregnant Rat Myometrium Biol Reprod, April 1, 2004; 70(4): 986 - 992. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S.R. Sooranna, Y. Lee, L.U. Kim, A.R. Mohan, P.R. Bennett, and M.R. Johnson Mechanical stretch activates type 2 cyclooxygenase via activator protein-1 transcription factor in human myometrial cells Mol. Hum. Reprod., February 1, 2004; 10(2): 109 - 113. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| Visit Other APS Journals Online |
