Am J Physiol Cell Physiol Information on EB 2010
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Am J Physiol Cell Physiol (March 20, 2002). doi:10.1152/ajpcell.00566.2001
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
283/2/C489    most recent
00566.2001v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Le, T. L.
Right arrow Articles by Stow, J. L.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Le, T. L.
Right arrow Articles by Stow, J. L.

Articles in PresS, published online ahead of print March 20, 2002
Am J Physiol Cell Physiol, 10.1152/ajpcell.00566.2001
Submitted on November 26, 2001
Accepted on March 14, 2002

Protein kinase C regulates both the endocytosis and recycling of E-cadherin

Tam Luan Le1, Shannon R. Joseph2, Alpha S. Yap3, and Jennifer L. Stow1*

1 Institute for Molecular Bioscience, University of Queensland, Brisbane, Queensland, Australia; Department of Biochemistry, University of Queensland, Brisbane, Queensland, Australia
2 Institute for Molecular Bioscience, University of Queensland, Brisbane, Queensland, Australia
3 Institute for Molecular Bioscience, University of Queensland, Brisbane, Queensland, Australia; Department of Physiology and Pharmacology, University of Queensland, Brisbane, Queensland, Australia

* To whom correspondence should be addressed. E-mail: j.stow{at}imb.uq.edu.au.

E-cadherin is a major component of adherens junctions in epithelial cells. We have previously shown that a pool of cell surface E-cadherin is constitutively internalized and recycled back to the surface. In the present study, we investigated the potential role of protein kinase C (PKC) in regulating the trafficking of surface E-cadherin in MDCK cells. Using surface biotinylation and immunofluorescence, we found that treatment of cells with phorbol esters increased the rate of endocytosis of E-cadherin, resulting in accumulation of E-cadherin in apically-localized early or recycling endosomes. The recycling of E-cadherin back to the surface was also decreased in the presence of phorbol esters. Phorbol ester-induced endocytosis of E-cadherin was blocked by specific inhibitors, implicating novel PKC isozymes, such as PKC-{epsilon} in this pathway . PKC activation led to changes in the actin cytoskeleton facilitating E-cadherin endocytosis. Depolymerization of actin increased endocytosis of E-cadherin while the PKC-induced uptake of E-cadherin was blocked by the actin stabilizer jasplakinolide. Our findings show that PKC regulates vital steps of E-cadherin trafficking, its endocytosis and its recycling.




This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
M. A. McGill, S. E. Dho, G. Weinmaster, and C. J. McGlade
Numb Regulates Post-endocytic Trafficking and Degradation of Notch1
J. Biol. Chem., September 25, 2009; 284(39): 26427 - 26438.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
L. Ivanova, M. J. Butt, and D. G. Matsell
Mesenchymal transition in kidney collecting duct epithelial cells
Am J Physiol Renal Physiol, May 1, 2008; 294(5): F1238 - F1248.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. L. Torgersen, S. Walchli, S. Grimmer, S. S. Skanland, and K. Sandvig
Protein Kinase C{delta} Is Activated by Shiga Toxin and Regulates Its Transport
J. Biol. Chem., June 1, 2007; 282(22): 16317 - 16328.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
D. M. Bryant, M. C. Kerr, L. A. Hammond, S. R. Joseph, K. E. Mostov, R. D. Teasdale, and J. L. Stow
EGF induces macropinocytosis and SNX1-modulated recycling of E-cadherin
J. Cell Sci., May 15, 2007; 120(10): 1818 - 1828.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
C.-Y. Yu, J.-Y. Chen, Y.-Y. Lin, K.-F. Shen, W.-L. Lin, C.-L. Chien, M. B.A. ter Beest, and T.-S. Jou
A Bipartite Signal Regulates the Faithful Delivery of Apical Domain Marker Podocalyxin/Gp135
Mol. Biol. Cell, May 1, 2007; 18(5): 1710 - 1722.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
J. G. Lock and J. L. Stow
Rab11 in Recycling Endosomes Regulates the Sorting and Basolateral Transport of E-Cadherin
Mol. Biol. Cell, April 1, 2005; 16(4): 1744 - 1755.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
J. A. Guttman, Y. Takai, and A. W. Vogl
Evidence That Tubulobulbar Complexes in the Seminiferous Epithelium Are Involved with Internalization of Adhesion Junctions
Biol Reprod, August 1, 2004; 71(2): 548 - 559.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
S. Iyer, D. M. Ferreri, N. C. DeCocco, F. L. Minnear, and P. A. Vincent
VE-cadherin-p120 interaction is required for maintenance of endothelial barrier function
Am J Physiol Lung Cell Mol Physiol, June 1, 2004; 286(6): L1143 - L1153.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
C. N. Wrobel, J. Debnath, E. Lin, S. Beausoleil, M. F. Roussel, and J. S. Brugge
Autocrine CSF-1R activation promotes Src-dependent disruption of mammary epithelial architecture
J. Cell Biol., April 26, 2004; 165(2): 263 - 273.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
X. Fang, H. Ji, S.-W. Kim, J.-I. Park, T. G. Vaught, P. Z. Anastasiadis, M. Ciesiolka, and P. D. McCrea
Vertebrate development requires ARVCF and p120 catenins and their interplay with RhoA and Rac
J. Cell Biol., April 12, 2004; 165(1): 87 - 98.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
J. L. Stow
ICAT is a multipotent inhibitor of {beta}-catenin. Focus on "Role for ICAT in {beta}-catenin-dependent nuclear signaling and cadherin functions"
Am J Physiol Cell Physiol, April 1, 2004; 286(4): C745 - C746.
[Full Text] [PDF]

Home page
 Molecular Cancer TherapeuticsHome page
S. K. Green, G. Francia, C. Isidoro, and R. S. Kerbel
Antiadhesive antibodies targeting E-cadherin sensitize multicellular tumor spheroids to chemotherapy in vitro
Mol. Cancer Ther., February 1, 2004; 3(2): 149 - 159.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
W. Lee-Kwon, J. H. Kim, J. W. Choi, K. Kawano, B. Cha, D. A. Dartt, D. Zoukhri, and M. Donowitz
Ca2+-dependent inhibition of NHE3 requires PKC{alpha} which binds to E3KARP to decrease surface NHE3 containing plasma membrane complexes
Am J Physiol Cell Physiol, December 1, 2003; 285(6): C1527 - C1536.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
M. A. Davis, R. C. Ireton, and A. B. Reynolds
A core function for p120-catenin in cadherin turnover
J. Cell Biol., November 10, 2003; 163(3): 525 - 534.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
K. Xiao, D. F. Allison, K. M. Buckley, M. D. Kottke, P. A. Vincent, V. Faundez, and A. P. Kowalczyk
Cellular levels of p120 catenin function as a set point for cadherin expression levels in microvascular endothelial cells
J. Cell Biol., November 10, 2003; 163(3): 535 - 545.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
L. Kusner and C. Carlin
Potential role for a novel AP180-related protein during endocytosis in MDCK cells
Am J Physiol Cell Physiol, November 1, 2003; 285(5): C995 - C1008.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
J. Yoo, A. Nichols, J. C. Song, J. Mammen, I. Calvo, R. T. Worrell, J. Cuppoletti, K. Matlin, and J. B. Matthews
Bryostatin-1 attenuates TNF-induced epithelial barrier dysfunction: role of novel PKC isozymes
Am J Physiol Gastrointest Liver Physiol, April 1, 2003; 284(4): G703 - G712.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Visit Other APS Journals Online
Copyright © 1976 by the American Physiological Society.