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1 Department of Pharmacology II, Osaka University Graduate School of Medicine, Suita, Osaka, Japan
2 Department of Pharmacology II, Osaka University Graduate School of Medicine, Suita, Osaka, Japan; Department of Veterinary Pharmacology, Osaka Prefecture University, Sakai, Osaka, Japan
3 Department of Pharmacology II, Osaka University Graduate School of Medicine, Suita, Osaka, Japan; Department of Ophthalmology, Osaka University Graduate School of Medicine, Suita, Osaka, Japan
* To whom correspondence should be addressed. E-mail: ykurachi{at}pharma2.med.osaka-u.ac.jp.
Kir5.1 is an inwardly rectifying K+ channel subunit whose functional role has not been fully elucidated. Expression and distribution of Kir5.1 in retina were examined using a specific polyclonal antibody. Kir5.1-immunoreactivity was detected in glial Muller cells and in some retinal neurons. In the Kir5.1-positive neurons the expression of glutamic acid decarboxylase GAD65) was detected, suggesting that they may be GABAergic-amacrine cells. In Muller cells, spots of Kir5.1-immunoreactivity distributed diffusely at the cell body and in the distal portions, where Kir4.1-immunoreactivity largely overlapped. In addition, Kir4.1-immunoreactivity without Kir5.1 was strongly concentrated at the endfoot of Muller cells facing the vitreous surface or in the processes surrounding vessels. The immunoprecipitant obtained from retina with anti-Kir4.1 antibody contained Kir5.1. These results suggest that heterotetrameric Kir4.1/Kir5.1-channels may exist in the cell body and distal portion of Muller cells, while homomeric Kir4.1-channels are clustered in the endfeet and surrounding vessels. It is possible that homomeric Kir4.1- and heteromeric Kir4.1/Kir5.1-channels play different functional roles in he K+-buffering action of Muller cells.
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