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Am J Physiol Cell Physiol (February 26, 2003). doi:10.1152/ajpcell.00556.2002
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Submitted on November 27, 2002
Accepted on February 11, 2003

Adenylyl Cyclase Expression and Modulation of cAMP in Rat Taste Cells

Tatjana Abaffy1*, Kristina R Trubey1, and Nirupa Chaudhari1

1 Department of Physiology and Biophysics, University of Miami, Miami, Fl, USA

* To whom correspondence should be addressed. E-mail: tabaffy{at}newssun.med.miami.edu.

cAMP is a second messenger implicated in sensory transduction for taste. The identity of adenylyl cyclase (AC) in taste cells has not been explored. We have employed RT-PCR to identify the AC isoforms present in taste cells and found that AC 4, 6 and 8 are expressed as mRNAs in taste tissue. These proteins are also expressed in a subset of taste cells as revealed by immunohistochemistry. Alterations of cAMP concentrations are associated with transduction of taste stimuli of several classes. The involvement of particular ACs in this modulation has not been investigated. We demonstrate that glutamate, which is a potent stimulus eliciting a taste quality termed umami, causes a decrease in cAMP in forskolin-treated taste cells. The potentiation of this response by inosine monophosphate, the lack of response to D-glutamate and the lack of response to umami stimuli in non-sensory lingual epithelium all suggest that the cAMP modulation represents umami taste transduction. Because cAMP down-regulation via ACs can be mediated through G{alpha}i proteins, we examined the colocalization of the detected ACs with G{alpha}i proteins and found that 66% of AC8 immuno-positive taste cells are also positive for gustducin, a taste specific G{alpha}i protein. Whether AC8 is directly involved in signal transduction of umami taste remains to be established.




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