Am J Physiol Cell Physiol AJP: Lung Cellular and Molecular Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Am J Physiol Cell Physiol (February 13, 2002). doi:10.1152/ajpcell.00555.2001
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
283/1/C212    most recent
00555.2001v2
00555.2001v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lim, I. J
Right arrow Articles by Longaker, M. T
Right arrow Search for Related Content
PubMed
Right arrow Articles by Lim, I. J
Right arrow Articles by Longaker, M. T

Articles in PresS, published online ahead of print February 13, 2002
Am J Physiol Cell Physiol, 10.1152/ajpcell.00555.2001
Submitted on December 3, 2001
Accepted on January 29, 2002

Fibroblast Collagen Secretion in Co-Culture with Keratinocytes: Normal Fibroblasts Secrete Collagen in a Keloid-Like Manner

Ivor J Lim1*, Toan-Thang Phan1, Boon-Huat Bay1, Robert Qi1, Hung-The Huynh1, Walter T Tan1, Seng-Teik Lee1, and Michael T Longaker1

1 Division of Plastic Surgery, Department of Surgery, National University of Singapore, Singapore, Singapore

* To whom correspondence should be addressed. E-mail: surlimi{at}nus.edu.sg.

Keloid scars represent a pathological response to cutaneous injury, reflecting a new set point between synthesis and degradation biased toward ECM collagen accumulation. Using a serum-free two-chamber co-culture model, we recently demonstrated a significant increase in normal fibroblast proliferation when co-cultured with keloid-derived keratinocytes. We hypothesized that similar keratinocyte-fibroblast interactions might influence fibroblast collagen production and examined conditioned media and cell lysate from co-culture for collagen I and III production by Western blot, allied with Northern analysis for procollagen I and III mRNA. Normal fibroblasts co-cultured with keloid keratinocytes produced increased soluble collagen I and III with a corresponding increase in pro-collagen I and III mRNA transcript levels. This was associated with decreased insoluble collagen from cell lysate. When keloid fibroblasts were co-cultured with keloid keratinocytes, both soluble and insoluble collagen were increased with associated procollagen III mRNA upregulation. Transmission electron microscopy of normal fibroblasts co-cultured with keloid keratinocytes showed an ECM appearance similar to in-vivo keloid tissue; an appearance not seen when normal fibroblasts were co-cultured with normal keratinocytes.




This article has been cited by other articles:


Home page
 Am. J. Pathol.Home page
T.-L. Tuan, P. Hwu, W. Ho, P. Yiu, R. Chang, A. Wysocki, and P. D. Benya
Adenoviral Overexpression and Small Interfering RNA Suppression Demonstrate That Plasminogen Activator Inhibitor-1 Produces Elevated Collagen Accumulation in Normal and Keloid Fibroblasts
Am. J. Pathol., November 1, 2008; 173(5): 1311 - 1325.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
T.-T. Phan, I. J. Lim, B. H. Bay, R. Qi, M. T. Longaker, S.-T. Lee, and H. Huynh
Role of IGF system of mitogens in the induction of fibroblast proliferation by keloid-derived keratinocytes in vitro
Am J Physiol Cell Physiol, April 1, 2003; 284(4): C860 - C869.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Visit Other APS Journals Online
Copyright © 1976 by the American Physiological Society.