Am J Physiol Cell Physiol Watch the video to learn how APS reaches out to developing nations.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Am J Physiol Cell Physiol (September 19, 2007). doi:10.1152/ajpcell.00554.2006
This Article
Right arrow Full Text (PDF)
Right arrow Supplemental Figure
Right arrow All Versions of this Article:
293/6/C1797    most recent
00554.2006v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Filosa, J. A.
Right arrow Articles by Gonzalez Bosc, L. V
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Filosa, J. A.
Right arrow Articles by Gonzalez Bosc, L. V
Submitted on October 30, 2006
Accepted on September 12, 2007

ACTIVITY-DEPENDENT NFATc3 NUCLEAR ACCUMULATION IN PERICYTES FROM CORTICAL PARENCHYMAL MICROVESSEL

Jessica Andrea Filosa1*, Mark T Nelson2, and Laura V Gonzalez Bosc3

1 Psychiatry, University of Cincinnati, Cincinnati, Ohio, United States
2 Pharmacology, University of Vermont, Burlington, Vermont, United States
3 Cell Biology and Physiology, University of New Mexico, Albuquerque, New Mexico, United States

* To whom correspondence should be addressed. E-mail: jessica.filosa{at}uc.edu.

The calcium-dependent transcription factor, NFATc3, is critical for embryonic vascular development and differentiation. Despite its potential importance, nothing is known about NFATc3 regulation in the brain microcirculation. In this study, we sought to investigate the role glutamate, possibly through astrocytic communication, plays in the control of NFATc3 regulation in pericytes from parenchymal microvessels. Coronal cortical slices from neonatal rats were subjected to electrical field stimulation or were treated with the metabotropic glutamate receptor agonist t-ACPD. NFATc3, glial fibrillary acidic protein (GFAP, astrocytic specific marker) and platelet-derived growth factor {beta}-receptor (PDGF-{beta} a pericyte specific marker) were detected by immunofluorescence. Electrical field stimulation induced NFATc3 nuclear accumulation in pericytes. This response was dependent on neuronal activity and group I metabotropic glutamate receptor activation. In addition, t-ACPD significantly increased NFATc3 nuclear accumulation in both astrocytes and pericytes. NFATc3 nuclear accumulation in pericytes was prevented when astrocytic function was abolished with the gliotoxin L-{alpha}-aminoadipate, or by inhibition of calcineurin, cyclooxygenase and nitric oxide synthase. This is the first study to report NFATc3 expression in pericytes from parenchymal microvessels and astrocytes from native tissue. Our results suggest a model by which glutamate, via mGluR activation, may regulate gene transcription in pluripotent vascular pericytes.




This article has been cited by other articles:


Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
S. de Frutos, C. H. Nitta, E. Caldwell, J. Friedman, and L. V. Gonzalez Bosc
Regulation of soluble guanylyl cyclase-{alpha}1 expression in chronic hypoxia-induced pulmonary hypertension: role of NFATc3 and HuR
Am J Physiol Lung Cell Mol Physiol, September 1, 2009; 297(3): L475 - L486.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
U. Panzer, O. M. Steinmetz, J.-E. Turner, C. Meyer-Schwesinger, C. von Ruffer, T. N. Meyer, G. Zahner, C. Gomez-Guerrero, R. M. Schmid, U. Helmchen, et al.
Resolution of renal inflammation: a new role for NF-{kappa}B1 (p50) in inflammatory kidney diseases
Am J Physiol Renal Physiol, August 1, 2009; 297(2): F429 - F439.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
S. de Frutos, L. Duling, D. Alo, T. Berry, O. Jackson-Weaver, M. Walker, N. Kanagy, and L. Gonzalez Bosc
NFATc3 is required for intermittent hypoxia-induced hypertension
Am J Physiol Heart Circ Physiol, May 1, 2008; 294(5): H2382 - H2390.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Visit Other APS Journals Online
Copyright © 1977 by the American Physiological Society.