Am J Physiol Cell Physiol Ad Instruments
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Am J Physiol Cell Physiol (April 24, 2002). doi:10.1152/ajpcell.00545.2001
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
283/3/C822    most recent
00545.2001v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Inuyama, M.
Right arrow Articles by Sawada, Y.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Inuyama, M.
Right arrow Articles by Sawada, Y.

Articles in PresS, published online ahead of print April 24, 2002
Am J Physiol Cell Physiol, 10.1152/ajpcell.00545.2001
Submitted on November 16, 2001
Accepted on April 18, 2002

Characteristics of L-lactic acid transport in basal membrane vesicles of human placental syncytiotrophoblast

Masako Inuyama1, Fumihiko Ushigome1, Akiko Emoto1, Noriko Koyabu1, Shoji Satoh2, Kiyomi Tsukimori2, Hitoo Nakano2, Hisakazu Ohtani1, and Yasufumi Sawada1*

1 Graduate School of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan
2 Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan

* To whom correspondence should be addressed. E-mail: sawada{at}phar.kyushu-u.ac.jp.

The characteristics of L-lactic acid transport across the trophoblast basal membrane were investigated and compared with those across the brush-border membrane using membrane vesicles isolated from human placenta. The uptake of [14C]L-lactic acid into basal membrane vesicles was Na+-independent, and an uphill transport was observed in the presence of a pH gradient ([H+]out > [H+]in). [14C]L-Lactic acid uptake exhibited saturation kinetics with a Kt value of 5.89 ± 0.68 mM in the presence of a pH gradient. p-Chloromercuribenzenesulfonate and {alpha}-cyano-4-hydroxycinnamate inhibited the initial uptake, whereas phloretin or 4,4'-diisothiocyanostilbene-2,2'-disulfonate did not. Mono- and dicarboxylic acids suppressed the initial uptake. In conclusion, L-lactic acid transport in the basal membrane is H+-dependent and Na+-independent, as is also the case for the brush-border membrane transport, and its characteristics resemble those of monocarboxylic acid transporters. However, there were several differences in the effects of inhibitors between basal and brush-border membrane vesicles, suggesting that the transporter(s) involved in L-lactic acid transport in the basal membrane of placental trophoblast may differ from those in the brush-border membrane.




This article has been cited by other articles:


Home page
 Reproductive SciencesHome page
P. Settle, C. P. Sibley, I. M. Doughty, T. Johnston, J. D. Glazier, T. L. Powell, T. Jansson, and S. W. D'Souza
Placental Lactate Transporter Activity and Expression in Intrauterine Growth Restriction
Reproductive Sciences, July 1, 2006; 13(5): 357 - 363.
[Abstract] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
K. Lahjouji, I. Elimrani, J. Lafond, L. Leduc, I. A. Qureshi, and G. A. Mitchell
L-Carnitine transport in human placental brush-border membranes is mediated by the sodium-dependent organic cation transporter OCTN2
Am J Physiol Cell Physiol, August 1, 2004; 287(2): C263 - C269.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
R. Mengual, K. el Abida, N. Mouaffak, M. Rieu, and M. Beaudry
Pyruvate shuttle in muscle cells: high-affinity pyruvate transport sites insensitive to trans-lactate efflux
Am J Physiol Endocrinol Metab, December 1, 2003; 285(6): E1196 - E1204.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Visit Other APS Journals Online
Copyright © 1976 by the American Physiological Society.