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Am J Physiol Cell Physiol (December 26, 2007). doi:10.1152/ajpcell.00537.2007
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Submitted on November 11, 2007
Accepted on December 20, 2007

ALKALINE pH- AND cAMP-INDUCED V-ATPase MEMBRANE ACCUMULATION IS MEDIATED BY PROTEIN KINASE A IN EPIDIDYMAL CLEAR CELLS

Nuria Pastor-Soler1*, Kenneth R Hallows2, Christy Smolak3, Fan Gong3, Dennis Brown4, and Sylvie Breton5

1 Medicine, Renal-Electrolyte Division, U. of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, United States; Cell Biology and Physiology, Center for Research in Reproductive Physiology, U. of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, United States
2 Cell Biology and Physiology, U. of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, United States; Medicine, Renal-Electrolyte Division, U. of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, United States
3 Medicine, Renal-Electrolyte Division, U. of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, United States
4 Program in Membrane Biology, Massachusetts General Hospital East, Boston, Massachusetts, United States; Center for Systems Biology, Massachusetts General Hospital, Boston, Massachusetts, United States; Medicine, Harvard Medical School, Boston, Massachusetts, United States
5 Program in Membrane Biology, Massachusetts General Hospital East, Boston, Massachusetts, United States; Center for Systems Biology, Massachusetts General Hospital, Boston, Massachusetts, United States; Harvard Medical School, Boston, Massachusetts, United States

* To whom correspondence should be addressed. E-mail: PastorN{at}dom.pitt.edu.

In the epididymis, low luminal bicarbonate and acidic pH maintain sperm quiescent during maturation and storage. The vacuolar H+-ATPase (V-ATPase) in epididymal clear cells plays a major role in luminal acidification. We have shown previously that cAMP, luminal alkaline pH, and activation of the bicarbonate-regulated soluble adenylyl cyclase (sAC) induce V-ATPase apical accumulation in these cells, thereby stimulating proton secretion into the epididymal lumen. Here we examined whether protein kinase A (PKA) is involved in this response. Confocal immunofluorescence labeling on rat epididymis perfused in vivo showed that at luminal acidic pH (6.5), V-ATPase was distributed between short apical microvilli and sub-apical endosomes. The specific PKA activator, N6-monobutyryl-3'-5-cyclic monophosphate (6-MB-cAMP, 1 mM), induced elongation of apical microvilli and accumulation of V-ATPase in these structures. The PKA inhibitor myristoylated-PKI (mPKI, 10 µM) inhibited the apical accumulation of V-ATPase induced by 6-MB-cAMP. Perfusion at pH 6.5 with 8-pCPT-2'-O-methyl-cAMP (8CPT-2-O-Me-cAMP; 10 µM), an activator of the exchange protein activated by cAMP (Epac), did not induce V-ATPase apical accumulation. When applied at a higher concentration (100 µM), 8CPT-2-O-Me-cAMP induced V-ATPase apical accumulation, but this effect was completely inhibited by mPKI suggesting crossover effects on the PKA pathway with this compound at high concentrations. Importantly, the physiologically relevant alkaline pH-induced apical V-ATPase accumulation was completely inhibited by pre-treatment with mPKI. We conclude that direct stimulation of PKA activity by cAMP is necessary and sufficient for the alkaline pH-induced accumulation of V-ATPase in clear cell apical microvilli.




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