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Am J Physiol Cell Physiol (April 18, 2002). doi:10.1152/ajpcell.00527.2001
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Articles in PresS, published online ahead of print April 18, 2002
Am J Physiol Cell Physiol, 10.1152/ajpcell.00527.2001
Submitted on November 5, 2001
Accepted on April 8, 2002

Sarcoplasmic reticulum Ca2+ pump mRNA stability in cardiac and smooth muscle: role of the 3'-untranslated region

Christine M. Misquitta1, James Mwanjewe2, Lin Nie3, and Ashok K. Grover2*

1 Biology, McMaster University, Hamilton, Ontario, Canada; Biology, McMaster University, Hamilton, Ontario, Canada
2 Medicine, McMaster University, Hamilton, Ontario, Canada
3 Medicine, McMaster University, Hamilton, Ontario, Canada; Biology, McMaster University, Hamilton, Ontario, Canada

* To whom correspondence should be addressed. E-mail: groverak{at}mcmaster.ca.

Stomach smooth muscle (SSM) and left ventricular muscle (LVM) express the sarcoplasmic reticulum Ca2+ pump (SERCA) gene SERCA2. Alternative splicing yields two major isoforms: SERCA2a in LVM and slow twitch muscle, and SERCA2b in SSM and most other tissues. The splices have different 3'-untranslated regions (UTR) and also encode proteins that differ slightly in their C-terminal domains. SERCA2 transcription rates are similar in the two tissues, yet LVM has a much higher level of SERCA2 mRNA than SSM. To understand the control of SERCA2 RNA expression, we inhibited transcription and showed that the half-life of SERCA2 mRNA is significantly longer (p < 0.05) in primary cultures of LVM cells than in SSM cells. Nuclear SERCA2 mRNA levels were also higher in LVM than in SSM. In vitro decay assays using synthetic RNA corresponding to the 3'-UTR of SERCA2a and 2b showed that: (a) nuclear extracts produced a faster decay of SERCA2 RNA than cytoplasmic extracts, (b) nuclear extracts produced a faster of decay of SERCA2b than of 2a. This was also true if the full length native mRNA was used instead of the 3'-UTR RNA, and (c) SERCA2b decay by cytoplasmic extracts was faster for LVM than for SSM. We propose that nuclear decay is an initial step in the control of SERCA2 RNA abundance and that this control is maintained or modulated in the cytoplasm. We discuss how these control mechanisms may be part of a control switch in cardiac development and pathophysiology.




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