Am J Physiol Cell Physiol AJP: Cell Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Am J Physiol Cell Physiol (February 5, 2003). doi:10.1152/ajpcell.00510.2002
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
284/6/C1334    most recent
00510.2002v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kutcher, L. W
Right arrow Articles by Dedman, J. R
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kutcher, L. W
Right arrow Articles by Dedman, J. R
Submitted on November 5, 2002
Accepted on January 21, 2003

Nuclear CaMKII Inhibits Neuronal Differentiation of PC12 Cells Without Affecting MAPK or CREB Activation

Louis W Kutcher1*, Shirelyn R Beauman1, Eric I Gruenstein2, Marcia A Kaetzel3, and John R Dedman3

1 Molecular and Cellular Physiology, University of Cincinnati, Cincinnati, OH, USA
2 Molecular Genetics, University of Cincinnati, Cincinnati, OH, USA
3 Genome Sciences, University of Cincinnati, Cincinnati, OH, USA; Molecular and Cellular Physiology, University of Cincinnati, Cincinnati, OH, USA

* To whom correspondence should be addressed. E-mail: Louis.Kutcher{at}UC.edu.

Ca2+/calmodulin regulated protein kinase II (CaMKII) mediates many cellular events. The four CaMKII isoforms have numerous splice variants, three of which contain nuclear localization signals. Little is known about the role of nuclear-localized CaMKII in neuronal development. To study this process, PC12 cells were transfected to produce CaMKII targeted to either the cytoplasm or the nucleus, then treated with nerve growth factor (NGF). NGF triggers a signaling cascade (MAPK) that results in the differentiation of PC12 cells into a neuronal phenotype, marked by neurite outgrowth. The present study found that cells expressing nuclear targeted CaMKII failed to grow neurites, while cells expressing cytoplasmic CaMKII readily produced neurites. Inhibition of neuronal differentiation by nuclear CaMKII was independent of MAPK, as sustained Erk phosphorylation was not affected. Phosphorylation of CREB was also unaffected. Thus nuclear CaMKII modifies neuronal differentiation by a mechanism independent of MAPK and CREB activation.




This article has been cited by other articles:


Home page
 Evid Based Complement Alternat MedHome page
A. Aiello, E. Fattorusso, M. Menna, R. Vitalone, H. C. Schroder, and W. E. G. Muller
Mumijo Traditional Medicine: Fossil Deposits from Antarctica (Chemical Composition and Beneficial Bioactivity)
Evid. Based Complement. Altern. Med., November 7, 2008; (2008) nen072v1.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
K. K. Lu, S. E. Armstrong, R. Ginnan, and H. A. Singer
Adhesion-dependent activation of CaMKII and regulation of ERK activation in vascular smooth muscle
Am J Physiol Cell Physiol, November 1, 2005; 289(5): C1343 - C1350.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. M. Schmitt, G. A. Wayman, N. Nozaki, and T. R. Soderling
Calcium Activation of ERK Mediated by Calmodulin Kinase I
J. Biol. Chem., June 4, 2004; 279(23): 24064 - 24072.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Interv.Home page
L. C. Griffith, C. S. Lu, and X. X. Sun
CaMKII, an Enzyme on the Move: Regulation of Temporospatial Localization
Mol. Interv., October 1, 2003; 3(7): 386 - 403.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Visit Other APS Journals Online
Copyright © 1976 by the American Physiological Society.