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Am J Physiol Cell Physiol (June 5, 2002). doi:10.1152/ajpcell.00502.2001
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Articles in PresS, published online ahead of print June 5, 2002
Am J Physiol Cell Physiol, 10.1152/ajpcell.00502.2001
Submitted on October 18, 2001
Accepted on June 3, 2002

Apparent elastic modulus and hysteresis of skeletal muscle cells throughout differentiation

Amy M. Collinsworth1, Sarah Zhang1, William E. Kraus2, and George A. Truskey1*

1 Biomedical Engineering, Duke University, Durham, NC, USA
2 Medicine, Duke University Medical Center, Durham, NC, USA

* To whom correspondence should be addressed. E-mail: gtruskey{at}duke.edu.

The effect of differentiation on the transverse mechanical properties of mammalian myocytes were determined using atomic force microscopy. The apparent elastic modulus increased from 11.5 ± 1.3 kPa for undifferentiated myoblasts to 45.3 ± 4.0 kPa after eight days of differentiation (p<0.05). The relative contribution of viscosity as determined from the normalized hysteresis area ranged from 0.13 ± 0.02 to 0.21 ± 0.03, and did not change throughout differentiation. Myosin expression correlated with the apparent elastic modulus but neither myosin nor ß-tubulin was associated with hysteresis. Microtubules did not affect mechanical properties since treatment with colchicine did not alter the apparent elastic modulus or hysteresis. Treatment with cytochalasin D or 2,3-butanedione 2-monoxime (BDM) led to a significant reduction in the apparent elastic modulus but no change in hysteresis. In summary, skeletal muscle cells exhibited viscoelastic behavior that changed during differentiation, yielding an increase in the transverse elastic modulus. Major contributors to changes in the transverse elastic modulus during differentiation were actin and myosin.




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