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Articles in PresS, published online ahead of print May 22, 2002
Am J Physiol Cell Physiol, 10.1152/ajpcell.00493.2001
Submitted on October 13, 2001
Accepted on April 29, 2002
1 Anatomy and Physiology, Kansas State University, Manhattan, KS, USA
* To whom correspondence should be addressed. E-mail: bschultz{at}vet.ksu.edu.
This study focused on the role of sodium bicarbonate cotransporter (NBC1) in cAMP-stimulated ion transport in porcine vas deferens epithelium. Ion substitution experiments in modified Ussing chambers revealed that cAMP-mediated stimulation was dependent upon the presence of Na+, HCO3-, and Cl- for a full response. HCO3--dependent current was unaffected by acetazolamide, bumetanide or amiloride but was inhibited by basolateral 4,4'-diaiminostilbene-2-2'-disulfonic acid. Na+-driven, HCO3--dependent, stilbene inhibitable anion flux was observed across the basolateral membrane of selectively permeablized monolayers. Results of radiotracer flux studies suggest a DNDS-sensitive stoichiometry of 2 base equivalents per Na+. Antibodies raised against rat kidney NBC epitopes (rkNBC; a.a. 338-391 and a.a. 928-1035) identified a single band of approximately 145 kDa. RT-PCR detected NBC1 message in porcine vas deferens epithelia. These results demonstrate that vas deferens epithelial cells possess the proteins necessary for the vectoral transport of HCO3- and that these mechanisms are maintained in primary culture. Taken together, the results indicate that vas deferens epithelia play an active role in male fertility and have implications for our understanding of the relationship between cystic fibrosis and congenital bilateral absence of the vas deferens.
This article has been cited by other articles:
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F. Pierucci-Alves and B. D. Schultz Bradykinin-Stimulated Cyclooxygenase Activity Stimulates Vas Deferens Epithelial Anion Secretion In Vitro in Swine and Humans Biol Reprod, September 1, 2008; 79(3): 501 - 509. [Abstract] [Full Text] [PDF]
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T. M Hagedorn, R. W Carlin, and B. D Schultz Oxytocin and Vasopressin Stimulate Anion Secretion by Human and Porcine Vas Deferens Epithelia Biol Reprod, September 1, 2007; 77(3): 416 - 424. [Abstract] [Full Text] [PDF]
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 M. Wilschanski, A. Dupuis, L. Ellis, K. Jarvi, J. Zielenski, E. Tullis, S. Martin, M. Corey, L.-C. Tsui, and P. Durie Mutations in the Cystic Fibrosis Transmembrane Regulator Gene and In Vivo Transepithelial Potentials Am. J. Respir. Crit. Care Med., October 1, 2006; 174(7): 787 - 794. [Abstract] [Full Text] [PDF]
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 R. W. Carlin, R. L. Sedlacek, R. R. Quesnell, F. Pierucci-Alves, D. M. Grieger, and B. D. Schultz PVD9902, a porcine vas deferens epithelial cell line that exhibits neurotransmitter-stimulated anion secretion and expresses numerous HCO3- transporters Am J Physiol Cell Physiol, June 1, 2006; 290(6): C1560 - C1571. [Abstract] [Full Text] [PDF]
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R. W. Carlin, J. H. Lee, D. C. Marcus, and B. D. Schultz Adenosine Stimulates Anion Secretion Across Cultured and Native Adult Human Vas Deferens Epithelia Biol Reprod, March 1, 2003; 68(3): 1027 - 1034. [Abstract] [Full Text] [PDF]
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