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Am J Physiol Cell Physiol (February 20, 2002). doi:10.1152/ajpcell.00457.2001
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Articles in PresS, published online ahead of print February 20, 2002
Am J Physiol Cell Physiol, 10.1152/ajpcell.00457.2001
Submitted on September 25, 2001
Accepted on February 18, 2002

Rabbit retinal neurons and glia express a variety of ENaC/DEG subunits

Laura M Brockway1, Zhen-Hong Zhou2, James K Bubien2, Biljana Jovov2, Dale J Benos2, and Kent T Keyser1*

1 Physiological Optics, University of Alabama at Birmingham, Birmingham, AL, USA
2 Physiology and Biophysics, University of Alabama at Birmigham, Birmingham, AL, USA

* To whom correspondence should be addressed. E-mail: brockwaylm{at}cs.com.

Some members of the ENaC/DEG family of ion channels have been detected in mammalian brain. Therefore, we examined the RNA and protein expression of these channels in another part of the central nervous system; the rabbit retina. We next sought to demonstrate physiological evidence for an amiloride-sensitive current in Muller glia which, based on a previous study, are thought to express {alpha}-ENaC (Golestaneh et al, 2001). RT-PCR using retinal RNA revealed the presence of {alpha}-, ß-, {gamma}-, and {delta}-ENaC as well as ASIC1, ASIC2, ASIC3, and ASIC4. Immunohistochemical localization with antibodies against {alpha}-ENaC and ß-ENaC showed labeling in Muller cells and neurons, respectively. The presence of {alpha}-ENaC, ß-ENaC, and ASIC1 were detected by Western blotting. Cultured Muller cells were whole-cell patch clamped. These cells exhibited an inward Na+ current which was blocked by amiloride. These data both demonstrate for the first time the expression of a variety of ENaC and ASIC subunits in the rabbit retina as well as distinct cellular expression patterns of specific subunits in neurons and glia.




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