Am J Physiol Cell Physiol Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Am J Physiol Cell Physiol (February 13, 2002). doi:10.1152/ajpcell.00456.2001
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
283/1/C77    most recent
00456.2001v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Smart, M L
Right arrow Articles by Petrou, S
Right arrow Search for Related Content
PubMed
Right arrow Articles by Smart, M L
Right arrow Articles by Petrou, S

Articles in PresS, published online ahead of print February 13, 2002
Am J Physiol Cell Physiol, 10.1152/ajpcell.00456.2001
Submitted on September 24, 2001
Accepted on December 31, 1969

Pore formation is not associated with macroscopic redistribution of P2X7 receptors

M L Smart1, R G Panchal1, D N Bowser1, D A Williams1, and S Petrou1*

1 Physiology, The University of Melbourne, Parkvillle, Vic, Australia

* To whom correspondence should be addressed. E-mail: spetrou{at}unimelb.edu.au.

The present study examines whether changes in P2X7 receptor density precede formation of the cytolytic pore characteristic of this receptor. We fused P2X7 receptors with enhanced green fluorescent protein (EGFP) at the N or C termini (EGFP-P2X7 and P2X7-EGFP). Electrophysiological characterization in Xenopus oocytes revealed wildtype responses to ATP for GFP-tagged receptors. However, differences in sensitivity to ATP were apparent with the P2X7-EGFP receptor displaying a three-fold reduction in ATP sensitivity compared to control. Ethidium bromide uptake was used to measure cytolytic pore formation. Comparison of tagged receptors with wildtype in HEK-293 and COS-7 cells showed there was no significant difference in ethidium+ uptake suggesting that fusions with EGFP did not interfere with cytolytic pore formation. Confocal microscopy confirmed that tagged receptors localized to the plasmalemma. Simultaneous monitoring of EGFP and ethidium+ fluorescence revealed that changes in receptor distribution do not precede pore formation. We conclude that it is unlikely that large scale changes in P2X7 receptor density precede pore formation.




This article has been cited by other articles:


Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
R. Sluyter, A. N. Shemon, W. E. Hughes, R. O. Stevenson, J. G. Georgiou, G. D. Eslick, R. M. Taylor, and J. S. Wiley
Canine erythrocytes express the P2X7 receptor: greatly increased function compared with human erythrocytes
Am J Physiol Regulatory Integrative Comp Physiol, November 1, 2007; 293(5): R2090 - R2098.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
Y.-H. Feng, L. Wang, Q. Wang, X. Li, R. Zeng, and G. I. Gorodeski
ATP stimulates GRK-3 phosphorylation and {beta}-arrestin-2-dependent internalization of P2X7 receptor
Am J Physiol Cell Physiol, June 1, 2005; 288(6): C1342 - C1356.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
R. X. Faria, F. P. DeFarias, and L. A. Alves
Are second messengers crucial for opening the pore associated with P2X7 receptor?
Am J Physiol Cell Physiol, February 1, 2005; 288(2): C260 - C271.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
B. J. Wisnoskey, M. Estacion, and W. P. Schilling
Maitotoxin-induced cell death cascade in bovine aortic endothelial cells: divalent cation specificity and selectivity
Am J Physiol Cell Physiol, August 1, 2004; 287(2): C345 - C356.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
Z. A. Pfeiffer, M. Aga, U. Prabhu, J. J. Watters, D. J. Hall, and P. J. Bertics
The nucleotide receptor P2X7 mediates actin reorganization and membrane blebbing in RAW 264.7 macrophages via p38 MAP kinase and Rho
J. Leukoc. Biol., June 1, 2004; 75(6): 1173 - 1182.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Visit Other APS Journals Online
Copyright © 1976 by the American Physiological Society.