Am J Physiol Cell Physiol Journal of Applied Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Am J Physiol Cell Physiol (June 13, 2002). doi:10.1152/ajpcell.00445.2001
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
283/3/C839    most recent
00445.2001v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Weng, T.
Right arrow Articles by Wills, N. K.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Weng, T.
Right arrow Articles by Wills, N. K.

Articles in PresS, published online ahead of print June 13, 2002
Am J Physiol Cell Physiol, 10.1152/ajpcell.00445.2001
Submitted on September 17, 2001
Accepted on May 1, 2002

Oxidant and antioxidant modulation of chloride channels expressed in human retinal pigment epithelium

Tianxiang Weng1, Bernard F. Godley2, Guifang Jin3, Nancy J. Mangini4, Brian G. Kennedy5, Alan S. Yu6, and Nancy K. Wills7*

1 Departments of Physiology and Biophysics, University of Texas Medical Branch, Galveston, TX, USA
2 The Retina Foundation of the Southwest, and Department of Ophthalmology, University of Texas Southwestern, Dallas, TX, USA
3 Ophthalmology and Visual Sciences, University of Texas Medical Branch, Galveston, TX, USA
4 Department of Anatomy, Indiana University School of Medicine, Gary, IN, USA
5 Department of Physiology, Indiana University School of Medicine, Gary, IN, USA
6 Department of Medicine, Renal Division, Harvard Institutes of Medicine, Boston, MA, USA
7 Departments of Physiology and Biophysics, University of Texas Medical Branch, Galveston, TX, USA; Ophthalmology and Visual Sciences, University of Texas Medical Branch, Galveston, TX, USA

* To whom correspondence should be addressed. E-mail: nkwills{at}utmb.edu.

Retinal pigment epithelium (RPE) possesses regulated chloride channels that are crucial for transepithelial fluid and ion transport. At present, little is known about the molecular nature of chloride channels in human adult RPE (ha-RPE) or the effects of oxidative stress on membrane conductance properties. In the present study, we assessed ClC channel and cystic fibrosis transmembrane conductance regulator (CFTR) expression and membrane chloride conductance properties in ha-RPE cells. ClC-5, ClC-3, and ClC-2, and CFTR mRNA expression was confirmed using RT-PCR analysis and protein expression was detected using Western blot analysis and immunofluorescence microscopy. Whole cell recordings of primary cultures of ha-RPE showed an outwardly rectifying chloride current that was inhibited by the oxidant hydrogen peroxide (H2O2). The inhibitory effects of H2O2 were reduced in cultured human RPE cells that were incubated with precursors of glutathione synthesis or that were stably transfected to overexpress glutathione S-transferase. These findings indicate a possible role for ClC channels in human adult RPE cells and suggest possible redox modulation of human RPE chloride conductances.




This article has been cited by other articles:


Home page
 Physiol. Rev.Home page
H. C. Hartzell, Z. Qu, K. Yu, Q. Xiao, and L.-T. Chien
Molecular Physiology of Bestrophins: Multifunctional Membrane Proteins Linked to Best Disease and Other Retinopathies
Physiol Rev, April 1, 2008; 88(2): 639 - 672.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
J. J. Matsuda, M. S. Filali, K. A. Volk, M. M. Collins, J. G. Moreland, and F. S. Lamb
Overexpression of CLC-3 in HEK293T cells yields novel currents that are pH dependent
Am J Physiol Cell Physiol, January 1, 2008; 294(1): C251 - C262.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
M. Jin, J. Yaung, R. Kannan, S. He, S. J. Ryan, and D. R. Hinton
Hepatocyte Growth Factor Protects RPE Cells from Apoptosis Induced by Glutathione Depletion
Invest. Ophthalmol. Vis. Sci., November 1, 2005; 46(11): 4311 - 4319.
[Abstract] [Full Text] [PDF]

Home page
 PhysiologyHome page
C. Hartzell, Z. Qu, I. Putzier, L. Artinian, L.-T. Chien, and Y. Cui
Looking Chloride Channels Straight in the Eye: Bestrophins, Lipofuscinosis, and Retinal Degeneration
Physiology, October 1, 2005; 20(5): 292 - 302.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
O. Strauss
The Retinal Pigment Epithelium in Visual Function
Physiol Rev, July 1, 2005; 85(3): 845 - 881.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
D. Reigada and C. H. Mitchell
Release of ATP from retinal pigment epithelial cells involves both CFTR and vesicular transport
Am J Physiol Cell Physiol, January 1, 2005; 288(1): C132 - C140.
[Abstract] [Full Text] [PDF]

Home page
 JGPHome page
D. M. Browe and C. M. Baumgarten
Angiotensin II (AT1) Receptors and NADPH Oxidase Regulate Cl- Current Elicited by {beta}1 Integrin Stretch in Rabbit Ventricular Myocytes
J. Gen. Physiol., August 30, 2004; 124(3): 273 - 287.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Visit Other APS Journals Online
Copyright © 1976 by the American Physiological Society.