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1 Pathology, Emory University, Atlanta, GA, USA
* To whom correspondence should be addressed. E-mail: sbagnas{at}emory.edu.
We have identified a urea transporter from the mucosa of human colon, which has characteristics consistent with a Kidd antigen/UT-B urea transporter. This intestinal urea transporter encodes a 389 amino acid peptide, with a sequence identical to that previously reported for the UT-B urea transporter in erythrocytes. Expression of a UT-B 2 kb mRNA transcript, and of ~50 and ~98 kDa UT-B proteins is detected in human colonic mucosa by Northern and Western analysis. The UT-B protein is localized in the cell membrane and cytoplasm of the superficial intestinal epithelium and in the epithelial cells in the crypts. A 2 kb UT-B mRNA transcript, and the UT-B protein were also identified in the intestinal cell line CaCo2 cells. The trans-epithelial flux of 14C-urea was examined in CaCo2 cells growing on porous membrane support, and was significantly inhibited by phloretin, 1,3 dimethylurea, and thiourea, suggesting that the transfer of urea across the CaCo2 monolayer could be mediated, at least in part, by the UT-B urea transporter. We conclude that the Kidd antigen/UT-B urea transporter is physiologically expressed in human colon epithelium, where it could participate in the transport of urea across the colon mucosa.
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