Whole cell patch clamp recordings were made from cultured myenteric neurons taken from murine proximal colon. The micropipette contained Cs⁺ to remove K⁺ currents. Depolarization elicited a slowly activating time-dependent outward current (I_tdo), whereas, repolarization was followed by a slowly deactivating tail current (I_tail). I_tdo and I_tail were present in about 70% of neurons. We identified these currents as Cl^- currents (I_Cl), because changing the transmembrane Cl^- gradient altered the measured reversal potential (E_rev) of both I_tdo and I_tail with that for I_tail shifted close to the calculated E_Cl. I_Cl are calcium-activated Cl^- current (I_Cl(Ca)) because they were Ca²⁺ dependent. E_Cl , which was measured from the reversal potential of I_Cl(Ca) using a gramicidin perforated patch, was -33mV. This value is more positive than the resting membrane potential (-56.3 ± 2.7mV), suggesting myenteric neurons accumulate intracellular Cl^- ions. -Conotoxin GIVA (0.3µM), [N-type Ca²⁺ channel blocker] and niflumic acid (10µM), [known I_Cl(Ca) blocker], decreased the I_Cl(Ca). In conclusion, these neurons have Ca²⁺-activated Cl^- currents, which are activated by calcium entry through N-type calcium channels. These currents likely regulate post spike frequency adaptation.