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Articles in PresS, published online ahead of print February 6, 2002
Am J Physiol Cell Physiol, 10.1152/ajpcell.00432.2001
Submitted on September 7, 2001
Accepted on January 29, 2002
1 Medicine, UCLA, Los Angeles, CA, USA
2 Medicine, UCLA, Los Angeles, CA, USA; Medicine, UCLA, Los Angeles, CA, USA
* To whom correspondence should be addressed. E-mail: erozengurt{at}mednet.ucla.edu.
We determined the effect of aromatic amino acid stimulation of the human extracellular Ca2+-sensing receptor (CaR) on intracellular Ca2+ concentration ([Ca2+]i) in single HEK 293 cells. Addition of L-phenylalanine or L-tryptophan (at 5 mM) induced [Ca2+]i oscillations from a resting state that was quiescent at 1.8 mM extracellular Ca2+ concentration ([Ca2+]e). Each [Ca2+]i peak returned to base-line values and the average oscillation frequency was ~1/min at 37°C. Oscillations were not induced or sustained if the [Ca2+]e was reduced to 0.5 mM, even in the continued presence of amino acid. Average oscillation frequency in response to an increase in [Ca2+]e (from 1.8 mM to 2.5 - 5 mM) was much higher (~4/min) than that induced by aromatic amino acids. Oscillations in response to [Ca2+]e were sinusoidal whereas those induced by amino acids were transient. Thus, both amino acids and Ca2+, acting through the same CaR, produce oscillatory increases in [Ca2+]i but the resultant oscillation pattern and frequency allows the cell to discriminate which agonist is bound to the receptor.
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