| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1 Departamento de Ciencias Fisiologicas, Pontificia Universidad Catolica de Chile, Santiago, Chile
2 Departamento de Quimica y Biologia, Universidad de Santiago de Chile, Santiago, Chile
* To whom correspondence should be addressed. E-mail: bcbranes{at}genes.bio.puc.cl.
The volume of the oviductal fluid fluctuates during the estrous cycle suggesting that water availability is under hormonal control. It has been postulated that sex-steroid hormones may regulate aquaporin (AQP) channels involved in water movement across cell membranes. Using a functional assay (oocytes of enopus laevis), we demonstrated that the rat oviductal epithelium contains mRNAs coding for water channels and we identified by RT-PCR the mRNAs for AQPs 5, 8 and 9, but not for AQP2 and AQP3. The immunoreactivity for AQPs 5, 8 and 9 was localized only in epithelial cells of the oviduct. The distribution of AQPs 5 and 8 was mainly cytoplasmic while we confirmed, by confocal microscopy, that AQP9 localized to the apical plasma membrane. Staining of AQPs 5, 8 and 9 was lost after ovariectomy and only AQP9 immunoreactivity was restored after estradiol and/or progesterone treatments. The recovery of AQP9 reactivity after ovariectomy correlated with increased mRNA and protein levels following treatment with estradiol alone or progesterone administration after estradiol priming. Interestingly, progesterone administration after progesterone priming also induced AQP9 expression but without a change in mRNA levels. Levels of AQP9 varied along the estrous cycle with their highest levels during proestrus and estrus. These results indicate that steroid hormones regulate AQP9 expression at the mRNA and protein level, and that other ovarian signals are involved in the expression of AQPs 5 and 8. Thus, hormonal regulation of the type, and quantity of water channels in this epithelium might control water transport in the oviductal lumen.
This article has been cited by other articles:
|
|
 |
|
  M. T. Skowronski, T.-H. Kwon, and S. Nielsen Immunolocalization of Aquaporin 1, 5, and 9 in the Female Pig Reproductive System J. Histochem. Cytochem., January 1, 2009; 57(1): 61 - 67. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Wellejus, H. E. Jensen, S. Loft, and T. E. Jonassen Expression of Aquaporin 9 in Rat Liver and Efferent Ducts of the Male Reproductive System After Neonatal Diethylstilbestrol Exposure J. Histochem. Cytochem., May 1, 2008; 56(5): 425 - 432. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Parada-Bustamante, P. A Orihuela, M. Rios, P. A Navarrete-Gomez, C. A Cuevas, L. A Velasquez, M. J Villalon, and H. B Croxatto Catechol-O-Methyltransferase and Methoxyestradiols Participate in the Intraoviductal Nongenomic Pathway Through Which Estradiol Accelerates Egg Transport in Cycling Rats Biol Reprod, December 1, 2007; 77(6): 934 - 941. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Leung, A. Pang, W.-H. Yuen, Y.-L. Kwong, and E. W. C. Tse Relationship of expression of aquaglyceroporin 9 with arsenic uptake and sensitivity in leukemia cells Blood, January 15, 2007; 109(2): 740 - 746. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H.-F. Huang, R.-H. He, C.-C. Sun, Y. Zhang, Q.-X. Meng, and Y.-Y. Ma Function of aquaporins in female and male reproductive systems Hum. Reprod. Update, November 1, 2006; 12(6): 785 - 795. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| Visit Other APS Journals Online |
