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Am J Physiol Cell Physiol (April 2, 2003). doi:10.1152/ajpcell.00413.2002
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Submitted on September 6, 2002
Accepted on March 25, 2003

Activation of Ca2+-activated Cl- current by depolarizing steps in rabbit urethral interstitial cells

Mark A Hollywood1, Gerard P Sergeant1, Noel G McHale1, and Keith D Thornbury1*

1 Department of Physiology, Queen's University Belfast, Belfast, United Kingdom

* To whom correspondence should be addressed. E-mail: k.thornbury{at}qub.ac.uk.

Interstitial cells were isolated from strips of rabbit urethra for study using the amphotericin B perforated patch technique. Depolarizing steps to -30 mV, or greater, activated a Ca2+ current (ICa), followed by a Ca2+-activated Cl- current and, on stepping back to -80 mV, large Cl- tail currents were observed. Both currents were abolished when the cells were superfused with Ca2+-free bath solution, suggesting that Ca2+ influx was necessary for activation of the Cl- current. The Cl- current was also abolished when Ba2+ was substituted for Ca2+ in the bath or the cell was dialysed with EGTA (2 mM). The Cl- current was also reduced by cyclopiazonic acid, ryanodine, 2-aminoethoxydiphenyl borate (2APB) and xestospongin C, suggesting that Ca2+-induced Ca2+ release (CICR) involving both ryanodine- and IP3-receptors contributes to its activation.




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