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Articles in PresS, published online ahead of print October 23, 2001
Am J Physiol Cell Physiol, 10.1152/ajpcell.00412.2001
Submitted on August 22, 2001
Accepted on October 19, 2001
1 Pediatrics and Physiology, University of Arizona, Tucson, AZ, USA
* To whom correspondence should be addressed. E-mail: fghishan{at}peds.arizona.edu.
The current studies were designed to characterize type IIb sodium-phosphate cotransporter (NaPi-IIb) expression and to assess the effect of 1,25-(OH)2 vitamin D3 (vit-D3) on NaPi-IIb gene expression during rat ontogeny. Sodium-dependent Pi absorption by intestinal brush-border membrane vesicles (BBMV) decreases with age, and NaPi-IIb gene expression also decreases proportionally with age. Vit-D3 treatment increased intestinal BBMV Pi absorption by ~2.5 fold in suckling rats and by ~2.1 fold in adult rats. Vit-D3 treatment also increased NaPi-IIb mRNA abundance by ~2 fold in 14-day-old rats, but had no effect on mRNA expression in adults. Furthermore, in rat intestinal epithelial (RIE) cells, vit-D3 increased NaPi-IIb mRNA abundance, an effect that was abolished by actinomycin D. Additionally, human NaPi-IIb gene promoter activity in transiently transfected RIE cells showed ~1.6-fold increase after vit-D3 treatment. In conclusion, we demonstrate that the age-related decrease in intestinal Na/Pi absorption correlates with decreased NaPi-IIb mRNA expression. Our data also suggest that the vit-D3 effect on NaPi-IIb expression is at least partially mediated by gene transcription in suckling rats.
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