| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
Articles in PresS, published online ahead of print October 16, 2002
Am J Physiol Cell Physiol, 10.1152/ajpcell.00398.2002
Submitted on August 29, 2002
Accepted on October 7, 2002
1 Cellular and Molecular Physiology, Yale University, New Haven, CT, USA
* To whom correspondence should be addressed. E-mail: cecilia.canessa{at}yale.edu.
The purpose of this study is to examine the role of the serum- and glucocorticoid-induced kinase (SGK) in the activation of the epithelial sodium channel (ENaC) by aldosterone, vasopressin (AVP) and insulin. We employed a tetracycline-inducible system to control the expression of wild-type (SGKTwt), constitutively active (SGKTS425D), or inactive (SGKTK130M) SGK, in A6 cells independently of hormonal stimulation. The effect of SGK expression on ENaC activity was monitored by measuring transepithelial amiloride-sensitive short circuit current (Isc) of transfected A6 cell lines. Expression of SGKTwt or SGKTS425D and aldosterone stimulation have additive effects on Isc. Although SGK could play some role in the aldosterone response, our results suggest that other mechanisms take place. SGKTS425D abrogates the responses to AVP and insulin hence, in the signaling pathways of these hormones there is a shared step that is stimulated by SGK. Considering that AVP and insulin induce fusion of vesicles to the apical membrane, our results support the notion that SGK promotes incorporation of channels to the apical membrane.
This article has been cited by other articles:
|
|
 |
|
  M. M. Vasquez, S. B. Mustafa, A. Choudary, S. R. Seidner, and R. Castro Regulation of Epithelial Na+ Channel (ENaC) in the Salivary Cell Line SMG-C6 Experimental Biology and Medicine, May 1, 2009; 234(5): 522 - 531. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. F. Ackermann, K. M. Boini, H. Volkl, M. Bhandaru, P. M. Bareiss, L. Just, V. Vallon, K. Amann, D. Kuhl, Y. Feng, et al. SGK1-sensitive renal tubular glucose reabsorption in diabetes Am J Physiol Renal Physiol, April 1, 2009; 296(4): F859 - F866. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Naray-Fejes-Toth, C. Boyd, and G. Fejes-Toth Regulation of epithelial sodium transport by promyelocytic leukemia zinc finger protein Am J Physiol Renal Physiol, July 1, 2008; 295(1): F18 - F26. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Fejes-Toth, G. Frindt, A. Naray-Fejes-Toth, and L. G. Palmer Epithelial Na+ channel activation and processing in mice lacking SGK1 Am J Physiol Renal Physiol, June 1, 2008; 294(6): F1298 - F1305. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. G. Brown, M. Gallacher, R. E. Olver, and S. M. Wilson The regulation of selective and nonselective Na+ conductances in H441 human airway epithelial cells Am J Physiol Lung Cell Mol Physiol, May 1, 2008; 294(5): L942 - L954. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Tiwari, L. Nordquist, V. K. M. Halagappa, and C. A. Ecelbarger Trafficking of ENaC subunits in response to acute insulin in mouse kidney Am J Physiol Renal Physiol, July 1, 2007; 293(1): F178 - F185. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Y. Huang, K. M. Boini, H. Osswald, B. Friedrich, F. Artunc, S. Ullrich, J. Rajamanickam, M. Palmada, P. Wulff, D. Kuhl, et al. Resistance of mice lacking the serum- and glucocorticoid-inducible kinase SGK1 against salt-sensitive hypertension induced by a high-fat diet Am J Physiol Renal Physiol, December 1, 2006; 291(6): F1264 - F1273. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Sandu, F. Artunc, M. Palmada, R. Rexhepaj, F. Grahammer, A. Hussain, C. Yun, D. R. Alessi, and F. Lang Impaired intestinal NHE3 activity in the PDK1 hypomorphic mouse Am J Physiol Gastrointest Liver Physiol, November 1, 2006; 291(5): G868 - G876. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Lang, C. Bohmer, M. Palmada, G. Seebohm, N. Strutz-Seebohm, and V. Vallon (Patho)physiological Significance of the Serum- and Glucocorticoid-Inducible Kinase Isoforms. Physiol Rev, October 1, 2006; 86(4): 1151 - 1178. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Bhalla, R. Soundararajan, A. C. Pao, H. Li, and D. Pearce Disinhibitory pathways for control of sodium transport: regulation of ENaC by SGK1 and GILZ Am J Physiol Renal Physiol, October 1, 2006; 291(4): F714 - F721. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Grahammer, F. Artunc, D. Sandulache, R. Rexhepaj, B. Friedrich, T. Risler, J. A. McCormick, K. Dawson, J. Wang, D. Pearce, et al. Renal function of gene-targeted mice lacking both SGK1 and SGK3 Am J Physiol Regulatory Integrative Comp Physiol, April 1, 2006; 290(4): R945 - R950. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Y. Huang, K. M. Boini, B. Friedrich, M. Metzger, L. Just, H. Osswald, P. Wulff, D. Kuhl, V. Vallon, and F. Lang Blunted hypertensive effect of combined fructose and high-salt diet in gene-targeted mice lacking functional serum- and glucocorticoid-inducible kinase SGK1 Am J Physiol Regulatory Integrative Comp Physiol, April 1, 2006; 290(4): R935 - R944. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Alvarez de la Rosa, I. Gimenez, B. Forbush, and C. M. Canessa SGK1 activates Na+-K+-ATPase in amphibian renal epithelial cells Am J Physiol Cell Physiol, February 1, 2006; 290(2): C492 - C498. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Gorelik, Y. Zhang, D. Sanchez, A. Shevchuk, G. Frolenkov, M. Lab, D. Klenerman, C. Edwards, and Y. Korchev Aldosterone acts via an ATP autocrine/paracrine system: The Edelman ATP hypothesis revisited PNAS, October 18, 2005; 102(42): 15000 - 15005. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. F. Arteaga and C. M. Canessa Functional specificity of Sgk1 and Akt1 on ENaC activity Am J Physiol Renal Physiol, July 1, 2005; 289(1): F90 - F96. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Markadieu, R. Crutzen, D. Blero, C. Erneux, and R. Beauwens Hydrogen peroxide and epidermal growth factor activate phosphatidylinositol 3-kinase and increase sodium transport in A6 cell monolayers Am J Physiol Renal Physiol, June 1, 2005; 288(6): F1201 - F1212. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. A. McCormick, V. Bhalla, A. C. Pao, and D. Pearce SGK1: A Rapid Aldosterone-Induced Regulator of Renal Sodium Reabsorption Physiology, April 1, 2005; 20(2): 134 - 139. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Boyd and A. Naray-Fejes-Toth Gene regulation of ENaC subunits by serum- and glucocorticoid-inducible kinase-1 Am J Physiol Renal Physiol, March 1, 2005; 288(3): F505 - F512. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Vallon, P. Wulff, D. Y. Huang, J. Loffing, H. Volkl, D. Kuhl, and F. Lang Role of Sgk1 in salt and potassium homeostasis Am J Physiol Regulatory Integrative Comp Physiol, January 1, 2005; 288(1): R4 - R10. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Mies, V. Shlyonsky, A. Goolaerts, and S. Sariban-Sohraby Modulation of epithelial Na+ channel activity by long-chain n-3 fatty acids Am J Physiol Renal Physiol, October 1, 2004; 287(4): F850 - F855. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. A. de la Rosa, T. G. Paunescu, W. J. Els, S. I. Helman, and C. M. Canessa Mechanisms of Regulation of Epithelial Sodium Channel by SGK1 in A6 Cells J. Gen. Physiol., September 27, 2004; 124(4): 395 - 407. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. P. Thomas, R. W. Loftus, and K. Z. Liu AVP-induced VIT32 gene expression in collecting duct cells occurs via trans-activation of a CRE in the 5'-flanking region of the VIT32 gene Am J Physiol Renal Physiol, September 1, 2004; 287(3): F460 - F468. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Fouladkou, R. Alikhani-Koopaei, B. Vogt, S. Y. Flores, L. Malbert-Colas, M.-C. Lecomte, J. Loffing, F. J. Frey, B. M. Frey, and O. Staub A naturally occurring human Nedd4-2 variant displays impaired ENaC regulation in Xenopus laevis oocytes Am J Physiol Renal Physiol, September 1, 2004; 287(3): F550 - F561. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Markadieu, D. Blero, A. Boom, C. Erneux, and R. Beauwens Phosphatidylinositol 3,4,5-trisphosphate: an early mediator of insulin-stimulated sodium transport in A6 cells Am J Physiol Renal Physiol, August 1, 2004; 287(2): F319 - F328. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Coric, N. Hernandez, D. A. de la Rosa, D. Shao, T. Wang, and C. M. Canessa Expression of ENaC and serum- and glucocorticoid-induced kinase 1 in the rat intestinal epithelium Am J Physiol Gastrointest Liver Physiol, April 1, 2004; 286(4): G663 - G670. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. A. de la Rosa, T. Coric, N. Todorovic, D. Shao, T. Wang, and C. M Canessa Distribution and regulation of expression of serum- and glucocorticoid-induced kinase-1 in the rat kidney J. Physiol., September 1, 2003; 551(2): 455 - 466. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| Visit Other APS Journals Online |
