| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Articles in PresS, published online ahead of print June 20, 2002
Am J Physiol Cell Physiol, 10.1152/ajpcell.00363.2001
Submitted on July 31, 2001
Accepted on June 11, 2002
1 Departament de Medicina, Universitat de Barcelona, Institut d'Investigacions Biomediques August Pi i Sunyer (IDIBAPS), Hospital Clinic, Barcelona, Catalonia, Spain
2 Departament de Medicina, Universitat de Barcelona, Institut d'Investigacions Biomediques August Pi i Sunyer (IDIBAPS), Hospital Clinic, Barcelona, Catalonia, Spain; Departament de Medicina, Universitat de Barcelona, Servei d'Otorinolaringologia, Hospital Clinic, Barcelona, Catalonia, Spain
3 Departament de Medicina, Universitat de Barcelona, Institut d'Investigacions Biomediques August Pi i Sunyer (IDIBAPS), Hospital Clinic, Barcelona, Catalonia, Spain; Departament de Medicina, Universitat de Barcelona, Servei de Pneumologia i Al.lergia Respiratoria - Institut Clinic de Pneumologia i Cirurgia Toracica, Hospital Clinic, Barcelona, Catalonia, Spain
4 Laboratory of Signal Transduction, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina, USA
* To whom correspondence should be addressed. E-mail: lpujols{at}medicina.ub.es.
Alternative splicing of the human glucocorticoid receptor (GR) primary transcript generates two protein isoforms: GR
and GRß. We investigated the expression of both GR isoforms in healthy human cells and tissues. GR mRNA abundance (x106 cDNA copies/µg total RNA) was: brain (3.83 ± 0.80) > skeletal muscle > macrophages > lung > kidney > liver > heart > eosinophils > peripheral blood mononuclear cells (PBMCs) > nasal mucosa > neutrophils > colon (0.33 ± 0.04). GRß mRNA was much less expressed than GR mRNA. Its abundance (x103 cDNA copies/µg total RNA) was: eosinophils (1.55 ± 0.58) > PBMCs > liver 
skeletal muscle > kidney > macrophages > lung > neutrophils > brain nasal mucosa > heart (0.15 ± 0.08). GRß mRNA was not found in colon. While GR protein was detected in all cells and tissues, GRß was not detected in any specimen. Our results suggest that in physiological conditions the default splicing pathway is the one leading to GR. The alternative splicing event leading to GRß is minimally activated.
This article has been cited by other articles:
|
|
 |
|
  R. S. Sane, D. J. Buckley, A. R. Buckley, S. C. Nallani, and P. B. Desai Role of Human Pregnane X Receptor in Tamoxifen- and 4-Hydroxytamoxifen-Mediated CYP3A4 Induction in Primary Human Hepatocytes and LS174T Cells Drug Metab. Dispos., May 1, 2008; 36(5): 946 - 954. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Oerlemans, J. Vink, B. A C Dijkmans, Y. G Assaraf, M. van Miltenburg, J. van der Heijden, I. Ifergan, W. F Lems, R. J Scheper, G. J L Kaspers, et al. Sulfasalazine sensitises human monocytic/macrophage cells for glucocorticoids by upregulation of glucocorticoid receptor {alpha} and glucocorticoid induced apoptosis Ann Rheum Dis, October 1, 2007; 66(10): 1289 - 1295. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. J. Lewis-Tuffin, C. M. Jewell, R. J. Bienstock, J. B. Collins, and J. A. Cidlowski Human Glucocorticoid Receptor {beta} Binds RU-486 and Is Transcriptionally Active Mol. Cell. Biol., March 15, 2007; 27(6): 2266 - 2282. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L.-b. Li, D. Y. M. Leung, C. F. Hall, and E. Goleva Divergent expression and function of glucocorticoid receptor {beta} in human monocytes and T cells J. Leukoc. Biol., April 1, 2006; 79(4): 818 - 827. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Goleva, L.-b. Li, P. T. Eves, M. J. Strand, R. J. Martin, and D. Y. M. Leung Increased Glucocorticoid Receptor beta Alters Steroid Response in Glucocorticoid-insensitive Asthma Am. J. Respir. Crit. Care Med., March 15, 2006; 173(6): 607 - 616. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. J. Barnes Corticosteroid effects on cell signalling Eur. Respir. J., February 1, 2006; 27(2): 413 - 426. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. R. Pondugula, N. N. Raveendran, Z. Ergonul, Y. Deng, J. Chen, J. D. Sanneman, L. G. Palmer, and D. C. Marcus Glucocorticoid regulation of genes in the amiloride-sensitive sodium transport pathway by semicircular canal duct epithelium of neonatal rat Physiol Genomics, January 12, 2006; 24(2): 114 - 123. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Torrego, L. Pujols, J. Roca-Ferrer, J. Mullol, A. Xaubet, and C. Picado Glucocorticoid Receptor Isoforms {alpha} and {beta} in in Vitro Cytokine-induced Glucocorticoid Insensitivity Am. J. Respir. Crit. Care Med., August 15, 2004; 170(4): 420 - 425. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L Pujols, A Xaubet, J Ramirez, J Mullol, J Roca-Ferrer, A Torrego, J A Cidlowski, and C Picado Expression of glucocorticoid receptors {alpha} and {beta} in steroid sensitive and steroid insensitive interstitial lung diseases Thorax, August 1, 2004; 59(8): 687 - 693. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Gupta and B. J. Wagner Expression of the Functional Glucocorticoid Receptor in Mouse and Human Lens Epithelial Cells Invest. Ophthalmol. Vis. Sci., May 1, 2003; 44(5): 2041 - 2046. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| Visit Other APS Journals Online |
