Am J Physiol Cell Physiol AJP citation statistics
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Am J Physiol Cell Physiol (December 18, 2003). doi:10.1152/ajpcell.00360.2003
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
286/5/C1045    most recent
00360.2003v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Brown, R. C
Right arrow Articles by Davis, T. P
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Brown, R. C
Right arrow Articles by Davis, T. P
Submitted on August 26, 2003
Accepted on December 15, 2003

PROTECTION AGAINST HYPOXIA-INDUCED BLOOD-BRAIN BARRIER DISRUPTION: CHANGES IN INTRACELLULAR CALCIUM

Rachel C Brown1*, Karen S Mark1, Richard D Egleton1, and Thomas P Davis1

1 Department of Pharmacology, University of Arizona, Tucson, AZ, USA

* To whom correspondence should be addressed. E-mail: rachel.brown{at}uth.tmc.edu.

Tissue damage following stroke is partly due to disruption of the blood-brain barrier (BBB). Little is known about the role of calcium in modulating BBB disruption. We investigated the effect of hypoxic and aglycemic stress on BBB function and intracellular calcium levels. Bovine brain microvessel endothelial cells were treated with A23187 to increase intracellular calcium without hypoxia, or were treated with calcium chelator (BAPTA) or calcium channel blockers (Nifedipine or SKF 96365) and 6 hr hypoxia. A23187 alone did not increase paracellular permeability. Hypoxia increased intracellular calcium and hypoxia or hypoxia/aglycemia increased paracellular permeability. Treatment with Nifedipine and SKF 96365 increased intracellular calcium under normoglycemic conditions, instead of blocking calcium influx, and was protective against hypoxia-induced BBB disruption under normoglycemia. Protection by Nifedipine and SKF 96365 was not due to antioxidant properties of these compounds. These data indicate that increased intracellular calcium alone is not enough to disrupt the BBB. However, increased intracellular calcium after drug treatment and hypoxia suggests a potential mechanism for these drugs in BBB protection; Nifedipine and SKF 96365 plus hypoxic stress may trigger calcium-mediated signaling cascades altering BBB integrity.




This article has been cited by other articles:


Home page
 J. Neurosci.Home page
Y. K. Choi, J. H. Kim, W. J. Kim, H. Y. Lee, J. A. Park, S.-W. Lee, D.-K. Yoon, H. H. Kim, H. Chung, Y. S. Yu, et al.
AKAP12 Regulates Human Blood-Retinal Barrier Formation by Downregulation of Hypoxia-Inducible Factor-1{alpha}
J. Neurosci., April 18, 2007; 27(16): 4472 - 4481.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
S. Foroutan, J. Brillault, B. Forbush, and M. E. O'Donnell
Moderate-to-severe ischemic conditions increase activity and phosphorylation of the cerebral microvascular endothelial cell Na+-K+-Cl- cotransporter
Am J Physiol Cell Physiol, December 1, 2005; 289(6): C1492 - C1501.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
M. A. Fleegal, S. Hom, L. K. Borg, and T. P. Davis
Activation of PKC modulates blood-brain barrier endothelial cell permeability changes induced by hypoxia and posthypoxic reoxygenation
Am J Physiol Heart Circ Physiol, November 1, 2005; 289(5): H2012 - H2019.
[Abstract] [Full Text] [PDF]

Home page
 Pharmacol. Rev.Home page
B. T. Hawkins and T. P. Davis
The Blood-Brain Barrier/Neurovascular Unit in Health and Disease
Pharmacol. Rev., June 1, 2005; 57(2): 173 - 185.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Visit Other APS Journals Online
Copyright © 1976 by the American Physiological Society.