Am J Physiol Cell Physiol Journal of Applied Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Am J Physiol Cell Physiol (January 2, 2002). doi:10.1152/ajpcell.00307.2001
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
282/5/C1093    most recent
00307.2001v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (11)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sherwood, J. J
Right arrow Articles by Eddinger, T. J
Right arrow Search for Related Content
PubMed
Right arrow Articles by Sherwood, J. J
Right arrow Articles by Eddinger, T. J

Articles in PresS, published online ahead of print January 2, 2002
Am J Physiol Cell Physiol, 10.1152/ajpcell.00307.2001
Submitted on July 5, 2001
Accepted on December 31, 2001

Shortening Velocity and Myosin Heavy and Light Chain Isoform mRNA in Rabbit Arterial Smooth Muscle Cells

Jennifer J Sherwood1* and Thomas J Eddinger1

1 Biology, Marquette University, Milwaukee, WI, USA

* To whom correspondence should be addressed. E-mail: sherwood{at}physiology.med.uvm.edu.

In smooth muscle cells (SMCs) isolated from rabbit carotid, femoral and saphenous arteries, relative myosin isoform mRNA levels were measured in reverse transcriptase-polymerase chain reactions (RT-PCRs) to test for correlations between myosin isoform expression and unloaded shortening velocity. Unloaded shortening velocity and %SM2 and %MLC17b mRNA levels were not significantly different in single SMCs isolated from the luminal and adluminal regions of the carotid media. Saphenous artery SMCs shortened significantly faster (p < 0.05) than femoral SMCs, had more SM2 mRNA (p<0.05) than carotid SMCs, less MLC17b mRNA (p<0.001) and higher tissue levels of SMB mRNA (p<0.05) than carotid and femoral SMCs. No correlations were found between %SM2 and %MLC17b mRNA levels and unloaded shortening velocity in SMCs from these arteries. We have previously shown that MHC SM1/SM2, SMA/SMB and MLC17 a/b isoform mRNA levels correlate with protein expression for these isoforms in rabbit SM tissues. Thus, we interpret these results to suggest that: 1) SMC myosin isoform expression and unloaded shortening velocity do not vary with distance from the lumen of the carotid artery but do vary in arteries located longitudinally within the arterial tree, 2) MHC SM1/SM2 and/or MLC17 a/b isoform expression does not correlate with unloaded shortening velocity and 3) that intracellular expression of the MHC SM1/SM2 and MLC17 a/b isoforms is not co-regulated.




This article has been cited by other articles:


Home page
 Am. J. Physiol. Renal Physiol.Home page
J. A. Hypolite, S. Chang, E. LaBelle, G. J. Babu, M. Periasamy, A. J. Wein, and S. Chacko
Deletion of SM-B, the high ATPase isoform of myosin, upregulates the PKC-mediated signal transduction pathway in murine urinary bladder smooth muscle
Am J Physiol Renal Physiol, March 1, 2009; 296(3): F658 - F665.
[Abstract] [Full Text] [PDF]

Home page
 Proc Am Thorac SocHome page
R. Leguillette and A.-M. Lauzon
Molecular Mechanics of Smooth Muscle Contractile Proteins in Airway Hyperresponsiveness and Asthma
Proceedings of the ATS, January 1, 2008; 5(1): 40 - 46.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
T. J. Eddinger and D. P. Meer
Myosin II isoforms in smooth muscle: heterogeneity and function
Am J Physiol Cell Physiol, August 1, 2007; 293(2): C493 - C508.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
M. Basha, S. Chang, E. M. Smolock, R. S. Moreland, A. J. Wein, and S. Chacko
Regional differences in myosin heavy chain isoform expression and maximal shortening velocity of the rat vaginal wall smooth muscle
Am J Physiol Regulatory Integrative Comp Physiol, October 1, 2006; 291(4): R1076 - R1084.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
S. Han, J. E. Speich, T. J. Eddinger, K. M. Berg, A. S. Miner, C. Call, and P. H. Ratz
Evidence for absence of latch-bridge formation in muscular saphenous arteries
Am J Physiol Heart Circ Physiol, July 1, 2006; 291(1): H138 - H146.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
D. H. Damon
Sympathetic innervation promotes vascular smooth muscle differentiation
Am J Physiol Heart Circ Physiol, June 1, 2005; 288(6): H2785 - H2791.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
K.-E. Andersson and A. Arner
Urinary Bladder Contraction and Relaxation: Physiology and Pathophysiology
Physiol Rev, July 1, 2004; 84(3): 935 - 986.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
M. E. DiSanto, R. Stein, S. Chang, J. A. Hypolite, Y. Zheng, S. Zderic, A. J. Wein, and S. Chacko
Alteration in expression of myosin isoforms in detrusor smooth muscle following bladder outlet obstruction
Am J Physiol Cell Physiol, December 1, 2003; 285(6): C1397 - C1410.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
P. Karagiannis, G. J Babu, M. Periasamy, and F. V Brozovich
The Smooth Muscle Myosin Seven Amino Acid Heavy Chain Insert's Kinetic Role in the Crossbridge Cycle for Mouse Bladder
J. Physiol., March 1, 2003; 547(2): 463 - 473.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Visit Other APS Journals Online
Copyright © 1976 by the American Physiological Society.