| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1 Physiology and Biophysics, University of Texas Medical Branch, Galveston, Texas, USA
* To whom correspondence should be addressed. E-mail: galtenbe{at}utmb.edu.
Phosphorylation of the gap-junction protein connexin 43 (Cx43) by protein kinase C (PKC) decreases dye coupling in many cell types. We report an investigation of the regulation by PKC of Cx43 gap-junctional hemichannels (GJH) expressed in Xenopus laevis oocytes. The activity of GJH was assessed from the uptake of hydrophilic fluorescent probes. PKC inhibitors increased probe uptake in isolated oocytes expressing recombinant Cx43, indicating that the regulatory effect occurs at the hemichannel level. We identified by mutational analysis the C-terminal domain sequences involved in this response. We found that: (a) S368 is responsible for the regulation of Cx43 GJH solute permeability by PKC-mediated phosphorylation, (b) C-terminal-domain residues 253 to 270 and 288 to 359 are not necessary for the effect of PKC, and (c) the P-rich C-terminal region is not involved in the effect of phosphorylation by PKC. Our results demonstrate that S368 (but not S372) is involved in the regulation of Cx43 solute permeability by PKC-mediated phosphorylation, and we conclude that different molecular mechanisms underlie the regulation of Cx43 by intracellular pH and PKC-mediated phosphorylation.
This article has been cited by other articles:
|
|
 |
|
  M. A. Retamal, S. Yin, G. A. Altenberg, and L. Reuss Modulation of Cx46 hemichannels by nitric oxide Am J Physiol Cell Physiol, June 1, 2009; 296(6): C1356 - C1363. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Miura, T. Yano, K. Naitoh, M. Nishihara, T. Miki, M. Tanno, and K. Shimamoto {delta}-Opioid receptor activation before ischemia reduces gap junction permeability in ischemic myocardium by PKC-{varepsilon}-mediated phosphorylation of connexin 43 Am J Physiol Heart Circ Physiol, September 1, 2007; 293(3): H1425 - H1431. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Winter and K. A. Dora Spreading dilatation to luminal perfusion of ATP and UTP in rat isolated small mesenteric arteries J. Physiol., July 1, 2007; 582(1): 335 - 347. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K Boengler, R Schulz, and G Heusch Connexin 43 signalling and cardioprotection Heart, December 1, 2006; 92(12): 1724 - 1727. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Matsushita, H. Kurihara, M. Watanabe, T. Okada, T. Sakai, and A. Amano Alterations of Phosphorylation State of Connexin 43 during Hypoxia and Reoxygenation Are Associated with Cardiac Function J. Histochem. Cytochem., March 1, 2006; 54(3): 343 - 353. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Y. Boateng, S. S. Lateef, W. Mosley, T. J. Hartman, L. Hanley, and B. Russell RGD and YIGSR synthetic peptides facilitate cellular adhesion identical to that of laminin and fibronectin but alter the physiology of neonatal cardiac myocytes Am J Physiol Cell Physiol, January 1, 2005; 288(1): C30 - C38. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Valiunas, R. Mui, E. McLachlan, G. Valdimarsson, P. R. Brink, and T. W. White Biophysical characterization of zebrafish connexin35 hemichannels Am J Physiol Cell Physiol, December 1, 2004; 287(6): C1596 - C1604. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| Visit Other APS Journals Online |
