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Am J Physiol Cell Physiol (November 21, 2001). doi:10.1152/ajpcell.00291.2001
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Articles in PresS, published online ahead of print November 20, 2001
Am J Physiol Cell Physiol, 10.1152/ajpcell.00291.2001
Submitted on June 26, 2001
Accepted on November 16, 2001

Distribution of the ClC-2 chloride channel in rat and human epithelial tissues

Joanna Lipecka1, Moez Bali1, Annick Thomas2, Pascale Fanen3, Aleksander Edelman1*, and Janine Fritsch4

1 INSERM U. 467, Paris, France
2 INSERM U. 410, Paris, France
3 INSERM U. 468, Paris, France
4 INSERM U. 467, Paris, France; CNRS UPR 1524, Paris, France

* To whom correspondence should be addressed. E-mail: edelman{at}citi2.fr.

The ubiquitous ClC-2 Cl- channel is thought to contribute to epithelial Cl- secretion, but the distribution of the ClC-2 protein in human epithelia has not been investigated. We have studied the distribution of ClC-2 in adult human and rat intestine and airways by immunoblotting and confocal microscopy. In the rat, ClC-2 was present in the lateral membranes of villus enterocytes and was predominant at the basolateral membranes of luminal colon enterocytes. The expression pattern of ClC-2 in the human intestine differed significantly, since ClC-2 was mainly detected in a supranuclear compartment of colon cells. We found significant expression of ClC-2 at the apex of ciliated cells in both rat and human airways. These results show that the distribution of ClC-2 in airways is consistent with participation of ClC-2 channels in chloride secretion and indicate that extrapolation of results from studies of ClC-2 function in rat intestine to human intestine is not straightforward.




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