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Am J Physiol Cell Physiol (January 8, 2003). doi:10.1152/ajpcell.00287.2002
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Submitted on June 21, 2002
Accepted on January 3, 2003

Reactive oxygen species are important mediators of taurine release from skeletal muscle cells

Niels Ortenblad1*, Jette F Young1, Niels Oksbjerg1, Jacob H Nielsen1, and Ian H Lambert2

1 Department of Animal Product Quality, Danish Institute of Agricultural Sciences, Foulum, DK-8830, Denmark
2 Biochemical Department, The August Krogh Institute, Copenhagen, DK-2100, Denmark

* To whom correspondence should be addressed. E-mail: n.ortenblad{at}zoo.latrobe.edu.au.

The present study illustrates elements of the signal cascades involved in the activation of taurine efflux pathways in myotubes derived from skeletal muscle cells. Exposing primary skeletal muscle cells, loaded with 14C-taurine, to (i) hypotonic media, (ii) the PLA2 activator melittin, (iii) anoxia, or (iv) lysophosphatidyl choline (LPC) causes an increase in 14C-taurine release and a concomitant production of reactive oxygen species (ROS). The antioxidants butulated hydroxy toluene and Vitamin E inhibit the taurine efflux following cell swelling, anoxia and addition of LPC. The muscle cells possess two separate taurine efflux pathways, i.e., a swelling- and melittin-induced pathway that requires 5-lipoxygenase activity for activation and a LPC-induced pathway. The two pathways are distinguished by their opposing sensitivity towards the anion channel blocker DIDS and cholesterol. These data provides evidence for PLA2 products and ROS as key mediators of the signal cascade leading to taurine efflux in muscle.




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