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1 Institute of Cellular and Organismic Biology, Academia Sinica, Taipei, Taiwan; Department of Physical Therapy, Shu-Zen College of Medicine and Management, Kaohsiung, Taiwan
2 Institute of Cellular and Organismic Biology, Academia Sinica, Taipei, Taiwan; Graduate Institute of Life Sciences, National Defense Medical Center, Taipei, Taiwan
3 Department of Biology, University of Massachusetts, Amherst, Massachusetts, USA
* To whom correspondence should be addressed. E-mail: pphwang{at}gate.sinica.edu.tw.
The mammalian kidney excretes its metabolic acid load through the proton transporting cells, intercalated cells, in the distal nephron and collecting duct. Fish excrete acid through external organs, gill or skin, however the cellular function is still controversial. In this work, molecular and electrophysiological approaches were used to identify a novel cell type secreting acid in skin of zebrafish larvae. Among keratinocytes covering larval surface, novel proton-secreting ionocytes, proton pump (H+ ATPase)-rich cells, were identified to generate strong outward H+ flux. The present work demonstrates for the first time, using non-invasive technique, H+ secreting cells in an intact model animal, the zebrafish, showing it to be a suitable model to study the functions of vertebrate transporting epithelia in vivo.
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