Am J Physiol Cell Physiol Journal of Neurophysiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Am J Physiol Cell Physiol (October 15, 2003). doi:10.1152/ajpcell.00271.2003
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
286/2/C372    most recent
00271.2003v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by van Balkom, B. W
Right arrow Articles by Deen, P. M
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by van Balkom, B. W
Right arrow Articles by Deen, P. M
Submitted on June 30, 2003
Accepted on October 12, 2003

Role of the cytoplasmic termini in the sorting and shuttling of the Aquaporin-2 water channel

Bas W van Balkom1, Michael P Graat1, Marcel van Raak1, Erik Hofman1, Peter van der Sluijs2, and Peter M Deen1*

1 Cell Physiology, UMC St Radboud, NCMLS, Nijmegen, The Netherlands
2 Cell Biology, Utrecht University, Utrecht, The Netherlands

* To whom correspondence should be addressed. E-mail: p.deen{at}ncmls.kun.nl.

In mammals, the regulation of water homeostasis is mediated by the aquaporin-1 (AQP1) water channel, which localizes to the basolateral and apical membranes of the early nephron segment, and AQP2, which is translocated from intracellular vesicles to the apical membrane of collecting duct cells after vasopressin stimulation. Since a similar localization and regulation is observed in transfected Madin Darby Canine Kidney (MDCK) cells, we investigated which segments of AQP2 are important for its routing to forskolin-sensitive vesicles and the apical membrane through analysis of AQP1-AQP2 chimeras. AQP1 with the entire C-tail of AQP2 was constitutively localized in the apical membrane, whereas chimeras with shorter C-tail segments of AQP2 were localized in the apical and basolateral membrane. AQP1 with the N-tail of AQP2 was constitutively localized in both plasma membranes, whereas AQP1 with the N- and C-tail of AQP2 was sorted to intracellular vesicles and translocated to the apical membrane with forskolin. These data indicate that region N220-S229 is essential for localization of AQP2 in the apical membrane and that the N- and C-tail of AQP2 are essential for trafficking of AQP2 to intracellular vesicles and its shuttling to and from the apical membrane.




This article has been cited by other articles:


Home page
 J. Am. Soc. Nephrol.Home page
E. E. Tchekneva, Z. Khuchua, L. S. Davis, V. Kadkina, S. R. Dunn, S. Bachman, K. Ishibashi, E. M. Rinchik, R. C. Harris, M. M. Dikov, et al.
Single Amino Acid Substitution in Aquaporin 11 Causes Renal Failure
J. Am. Soc. Nephrol., October 1, 2008; 19(10): 1955 - 1964.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
H. J. Lu, T. Matsuzaki, R. Bouley, U. Hasler, Q.-H. Qin, and D. Brown
The phosphorylation state of serine 256 is dominant over that of serine 261 in the regulation of AQP2 trafficking in renal epithelial cells
Am J Physiol Renal Physiol, July 1, 2008; 295(1): F290 - F294.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
P. P. Shi, X. R. Cao, J. Qu, K. A. Volk, P. Kirby, R. A. Williamson, J. B. Stokes, and B. Yang
Nephrogenic diabetes insipidus in mice caused by deleting COOH-terminal tail of aquaporin-2
Am J Physiol Renal Physiol, May 1, 2007; 292(5): F1334 - F1344.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
T. Rai, S. Sasaki, and S. Uchida
Polarized trafficking of the aquaporin-3 water channel is mediated by an NH2-terminal sorting signal
Am J Physiol Cell Physiol, January 1, 2006; 290(1): C298 - C304.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
F. de Mattia, P. J.M. Savelkoul, E.-J. Kamsteeg, I. B.M. Konings, P. van der Sluijs, R. Mallmann, A. Oksche, and P. M.T. Deen
Lack of Arginine Vasopressin-Induced Phosphorylation of Aquaporin-2 Mutant AQP2-R254L Explains Dominant Nephrogenic Diabetes Insipidus
J. Am. Soc. Nephrol., October 1, 2005; 16(10): 2872 - 2880.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
M. A.J. Devonald and F. E. Karet
Renal Epithelial Traffic Jams and One-Way Streets
J. Am. Soc. Nephrol., June 1, 2004; 15(6): 1370 - 1381.
[Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Visit Other APS Journals Online
Copyright © 1976 by the American Physiological Society.