Am J Physiol Cell Physiol Watch the video to learn how APS reaches out to developing nations.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Am J Physiol Cell Physiol (October 31, 2001). doi:10.1152/ajpcell.00271.2001
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
282/3/C617    most recent
00271.2001v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Stamenovic, D.
Right arrow Articles by Wang, N.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Stamenovic, D.
Right arrow Articles by Wang, N.

Articles in PresS, published online ahead of print October 31, 2001
Am J Physiol Cell Physiol, 10.1152/ajpcell.00271.2001
Submitted on June 15, 2001
Accepted on October 19, 2001

Cell prestress. II. Contribution of microtubules

Dimitrije Stamenovic1*, Srboljub M Mijailovich2, Iva Marija Tolic-Norrelykke3, Jianxin Chen2, and Ning Wang2

1 Department of Biomedical Engineering, Boston University, Boston, Massachusetts, USA
2 Department of Environmental Health, Physiology Program, Harvard School of Public Health, Boston, Massachusetts, USA
3 Rugjer Boskovic Institute, Zagreb, Croatia (Hrvatska); Department of Environmental Health, Physiology Program, Harvard School of Public Health, Boston, Massachusetts, USA

* To whom correspondence should be addressed. E-mail: dimitrij{at}engc.bu.edu.

The tensegrity model hypothesizes that cytoskeleton-based microtubules (MTs) carry compression as they balance a portion of cell contractile stress. To test this hypothesis, traction force microscopy was used to measure traction at the interface of adhering human airway smooth muscle cells and a flexible polyacrylamide gel substrate. The prediction is that if MTs indeed balance a portion of the contractile stress, then, upon their disruption, the portion of the stress balanced by MTs would shift to the substrate, causing thereby an increase in traction. Measurements were done first in maximally activated cells (10 µM histamine) and then after microtubules were disrupted by colchicine (1 µM). It was found that following disruption of MTs the traction increased on average by ~13%. Since in the activated cells colchicine neither induced an increase in intracellular calcium nor an increase in the myosin light chain phosphorylation as shown previously, it was concluded that the observed increase in traction was a result of the load-shift from MTs to the substrate. In addition, the energy stored in the flexible substrate was calculated as the work done by the traction on the deformation of the substrate. This result was then utilized in an energetic analysis. It was assumed that cytoskeleton-based MTs are slender elastic rods that are supported laterally by intermediate filaments, and that MTs buckle as the cell contracts. Using the post-buckling equilibrium theory of Euler struts, it was found that the energy stored during buckling of MTs was quantitatively consistent with the measured increase in the substrate energy following disruption of MTs. This was another piece of evidence in support of the idea that MTs are intracellular compression bearing elements.




This article has been cited by other articles:


Home page
 J. Cell Sci.Home page
A. M. Goldyn, B. A. Rioja, J. P. Spatz, C. Ballestrem, and R. Kemkemer
Force-induced cell polarisation is linked to RhoA-driven microtubule-independent focal-adhesion sliding
J. Cell Sci., October 15, 2009; 122(20): 3644 - 3651.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
R. C. Geiger, C. D. Kaufman, A. P. Lam, G. R. S. Budinger, and D. A. Dean
Tubulin Acetylation and Histone Deacetylase 6 Activity in the Lung under Cyclic Load
Am. J. Respir. Cell Mol. Biol., January 1, 2009; 40(1): 76 - 82.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
K. Nagayama and T. Matsumoto
Contribution of actin filaments and microtubules to quasi-in situ tensile properties and internal force balance of cultured smooth muscle cells on a substrate
Am J Physiol Cell Physiol, December 1, 2008; 295(6): C1569 - C1578.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
C. S. Chen
Mechanotransduction - a field pulling together?
J. Cell Sci., October 15, 2008; 121(20): 3285 - 3292.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
C. P. Brangwynne, F. C. MacKintosh, S. Kumar, N. A. Geisse, J. Talbot, L. Mahadevan, K. K. Parker, D. E. Ingber, and D. A. Weitz
Microtubules can bear enhanced compressive loads in living cells because of lateral reinforcement
J. Cell Biol., June 5, 2006; 173(5): 733 - 741.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
S. Ito, A. Majumdar, H. Kume, K. Shimokata, K. Naruse, K. R. Lutchen, D. Stamenovic, and B. Suki
Viscoelastic and dynamic nonlinear properties of airway smooth muscle tissue: roles of mechanical force and the cytoskeleton
Am J Physiol Lung Cell Mol Physiol, June 1, 2006; 290(6): L1227 - L1237.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
P. C. Georges and P. A. Janmey
Cell type-specific response to growth on soft materials
J Appl Physiol, April 1, 2005; 98(4): 1547 - 1553.
[Abstract] [Full Text] [PDF]

Home page
 PhysiologyHome page
B. P. Helmke
Molecular Control of Cytoskeletal Mechanics by Hemodynamic Forces
Physiology, February 1, 2005; 20(1): 43 - 53.
[Abstract] [Full Text] [PDF]

Home page
 J. Histochem. Cytochem.Home page
S. P. Evanko, W. T. Parks, and T. N. Wight
Intracellular Hyaluronan in Arterial Smooth Muscle Cells: Association with Microtubules, RHAMM, and the Mitotic Spindle
J. Histochem. Cytochem., December 1, 2004; 52(12): 1525 - 1536.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
D. Stamenovic, B. Suki, B. Fabry, N. Wang, J. J. Fredberg, and J. E. Buy
Rheology of airway smooth muscle cells is associated with cytoskeletal contractile stress
J Appl Physiol, May 1, 2004; 96(5): 1600 - 1605.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
S. S. An, B. Fabry, M. Mellema, P. Bursac, W. T. Gerthoffer, U. S. Kayyali, M. Gaestel, S. A. Shore, and J. J. Fredberg
Role of heat shock protein 27 in cytoskeletal remodeling of the airway smooth muscle cell
J Appl Physiol, May 1, 2004; 96(5): 1701 - 1713.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
S. Hu, J. Chen, B. Fabry, Y. Numaguchi, A. Gouldstone, D. E. Ingber, J. J. Fredberg, J. P. Butler, and N. Wang
Intracellular stress tomography reveals stress focusing and structural anisotropy in cytoskeleton of living cells
Am J Physiol Cell Physiol, November 1, 2003; 285(5): C1082 - C1090.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
S. J. Gunst and J. J. Fredberg
The first three minutes: smooth muscle contraction, cytoskeletal events, and soft glasses
J Appl Physiol, July 1, 2003; 95(1): 413 - 425.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
D. E. Ingber
Tensegrity I. Cell structure and hierarchical systems biology
J. Cell Sci., April 1, 2003; 116(7): 1157 - 1173.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
N. Wang, I. M. Tolic-Norrelykke, J. Chen, S. M. Mijailovich, J. P. Butler, J. J. Fredberg, and D. Stamenovic
Cell prestress. I. Stiffness and prestress are closely associated in adherent contractile cells
Am J Physiol Cell Physiol, March 1, 2002; 282(3): C606 - C616.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
J. P. Butler, I. M. Tolic-Norrelykke, B. Fabry, and J. J. Fredberg
Traction fields, moments, and strain energy that cells exert on their surroundings
Am J Physiol Cell Physiol, March 1, 2002; 282(3): C595 - C605.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Visit Other APS Journals Online
Copyright © 1976 by the American Physiological Society.