| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Articles in PresS, published online ahead of print August 14, 2002
Am J Physiol Cell Physiol, 10.1152/ajpcell.00266.2002
Submitted on June 7, 2002
Accepted on August 9, 2002
1 Physiology and Cell Biology, University of Nevada School of Medicine, Reno, NV, USA
* To whom correspondence should be addressed. E-mail: burt{at}physio.unr.edu.
Interstitial Cells of Cajal (ICC) are the pacemaker cells responsible for the generation and propagation of electrical slow waves in phasic muscles of the gastrointestinal (GI) tract. The pacemaker current that initiates each slow wave derives from a calcium-inhibited, voltage independent nonselective cation channel. This channel in ICC displays properties similar to that reported for the Transient Receptor Potential (TRP) family of non-selective cation channels, particularly those seen for TRPC3 and TRPC4. We have identified transcripts for TRPC4 in individually isolated ICC and have cloned the two alternatively spliced forms of TRPC4; TRPC4
and TRPC4ß from GI muscles. TRPC4ß is missing an 84 amino acid segment from the carboxy terminus. Expression of either form using the whole-cell patch clamp technique led to calcium-inhibited nonselective cation channels as determined by NMDG+ replacement experiments and BAPTA dialysis. Expression of TRPC4ß channels recorded at the whole cell level had characteristics similar to the nonselective cation current in ICC. The single channel conductance of TRPC4ß was determined to be 17.5pS. Application of calmidazolium to cells expressing TRPC4ß led to a significant increase in the inward current of these cells at both the whole cell and single channel level, and currents were sensitive to block by 10µM lanthanum, niflumic acid and DIDS. Comparison of the properties reported for the nonselective cation current in ICC and those identified here for TRPC4ß led us to conclude that a TRPC4-like current encodes the plasmalemmal pacemaker current in murine small intestine.
This article has been cited by other articles:
|
|
 |
|
  N. G. Jin, S. D. Koh, and K. M. Sanders Caffeine inhibits nonselective cationic currents in interstitial cells of Cajal from the murine jejunum Am J Physiol Cell Physiol, October 1, 2009; 297(4): C971 - C978. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Collin, R. Franconville, B. E. Ehrlich, and I. Llano Activation of Metabotropic Glutamate Receptors Induces Periodic Burst Firing and Concomitant Cytosolic Ca2+ Oscillations in Cerebellar Interneurons J. Neurosci., July 22, 2009; 29(29): 9281 - 9291. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Chen, T. Ordog, J. Chen, D. L. Young, M. R. Bardsley, D. Redelman, S. M. Ward, and K. M. Sanders Differential gene expression in functional classes of interstitial cells of Cajal in murine small intestine Physiol Genomics, November 14, 2007; 31(3): 492 - 509. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y.-K. Ju, Y. Chu, H. Chaulet, D. Lai, O. L. Gervasio, R. M. Graham, M. B. Cannell, and D. G. Allen Store-Operated Ca2+ Influx and Expression of TRPC Genes in Mouse Sinoatrial Node Circ. Res., June 8, 2007; 100(11): 1605 - 1614. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Zhu, J. Ye, and J. D. Huizinga Clotrimazole-sensitive K+ currents regulate pacemaker activity in interstitial cells of Cajal Am J Physiol Gastrointest Liver Physiol, June 1, 2007; 292(6): G1715 - G1725. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Nakayama, K. Shimono, H.-N. Liu, H. Jiko, N. Katayama, T. Tomita, and K. Goto Pacemaker phase shift in the absence of neural activity in guinea-pig stomach: a microelectrode array study J. Physiol., November 1, 2006; 576(3): 727 - 738. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. T. Kim, B. J. Kim, J. H. Lee, S. C. Kwon, D. S. Yeon, D. K. Yang, I. So, and K. W. Kim Involvement of calmodulin and myosin light chain kinase in activation of mTRPC5 expressed in HEK cells Am J Physiol Cell Physiol, April 1, 2006; 290(4): C1031 - C1040. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. R. Strege, C. E. Bernard, Y. Ou, S. J. Gibbons, and G. Farrugia Effect of mibefradil on sodium and calcium currents Am J Physiol Gastrointest Liver Physiol, August 1, 2005; 289(2): G249 - G253. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Zhu, A. Mucci, and J. D. Huizinga Inwardly rectifying chloride channel activity in intestinal pacemaker cells Am J Physiol Gastrointest Liver Physiol, April 1, 2005; 288(4): G809 - G821. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Yano, T. Ishikawa, H. Tsuda, K. Obara, and K. Nakayama Ionic mechanism for contractile response to hyposmotic challenge in canine basilar arteries Am J Physiol Cell Physiol, March 1, 2005; 288(3): C702 - C709. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Fantozzi, S. Zhang, O. Platoshyn, C. V. Remillard, R. T. Cowling, and J. X.-J. Yuan Hypoxia increases AP-1 binding activity by enhancing capacitative Ca2+ entry in human pulmonary artery endothelial cells Am J Physiol Lung Cell Mol Physiol, December 1, 2003; 285(6): L1233 - L1245. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. G. Shlykov, M. Yang, J. L. Alcorn, and B. M. Sanborn Capacitative Cation Entry in Human Myometrial Cells and Augmentation by hTrpC3 Overexpression Biol Reprod, August 1, 2003; 69(2): 647 - 655. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| Visit Other APS Journals Online |
