Am J Physiol Cell Physiol AJP citation statistics
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Am J Physiol Cell Physiol (September 24, 2008). doi:10.1152/ajpcell.00255.2008
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
295/6/C1625    most recent
00255.2008v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Shih, T.-H.
Right arrow Articles by Lin, L.-Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Shih, T.-H.
Right arrow Articles by Lin, L.-Y.
Submitted on May 14, 2008
Revised on August 11, 2008
Accepted on September 22, 2008

Ammonia excretion by the skin of zebrafish (Danio rerio) larvae

Tin-Han Shih1, Jiun-Lin Horng2, Pung-Pung Hwang3, and Li-Yih Lin1*

1 Department of Life Science
2 Academia Sinica
3 Institute of Cellular and Organismic Biology, Academia Sinica

* To whom correspondence should be addressed. E-mail: linly{at}ntnu.edu.tw.

The mechanism of ammonia excretion in freshwater teleosts is not well understood. In this study, scanning ion-selective electrode technique (SIET) was applied to measure H+ and NH4+ fluxes in specific cells on the skin of zebrafish larvae. NH4+ extrusion was relatively high in H+ pump-rich cells (HRCs), which were identified as the H+-secreting ionocyte in zebrafish. Minor NH4+ extrusion was also detected in keratinocytes and other types of ionocytes in larval skin. NH4+ extrusion from the skin was tightly linked to acid secretion. Increases in the external pH and buffer concentration (5 mM MOPS) diminished H+ and NH4+ gradients at the larval surface. Moreover, coupled decreases in NH4+ and H+ extrusion were found in larvae treated with an H+-pump inhibitor (bafilomycin A1) or H+-pump gene (atp6v1a) knockdown. Knockdown of Rhcg1 with morpholino-oligoneculeotides also decreased NH4+ excretion. This study demonstrates ammonia excretion in epithelial cells of larval skin through an acid-trapping mechanism, and provides direct evidence for the involvement of the H+ pump and an Rh glycoprotein (Rhcg1) in ammonia excretion.




This article has been cited by other articles:


Home page
 Am. J. Physiol. Cell Physiol.Home page
J.-L. Horng, P.-P. Hwang, T.-H. Shih, Z.-H. Wen, C.-S. Lin, and L.-Y. Lin
Chloride transport in mitochondrion-rich cells of euryhaline tilapia (Oreochromis mossambicus) larvae
Am J Physiol Cell Physiol, October 1, 2009; 297(4): C845 - C854.
[Abstract] [Full Text] [PDF]

Home page
 J. Exp. Biol.Home page
D. Weihrauch, M. P. Wilkie, and P. J. Walsh
Ammonia and urea transporters in gills of fish and aquatic crustaceans
J. Exp. Biol., June 1, 2009; 212(11): 1716 - 1730.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
M. H. Braun, S. L. Steele, M. Ekker, and S. F. Perry
Nitrogen excretion in developing zebrafish (Danio rerio): a role for Rh proteins and urea transporters
Am J Physiol Renal Physiol, May 1, 2009; 296(5): F994 - F1005.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
J.-L. Horng, L.-Y. Lin, and P.-P. Hwang
Functional regulation of H+-ATPase-rich cells in zebrafish embryos acclimated to an acidic environment
Am J Physiol Cell Physiol, April 1, 2009; 296(4): C682 - C692.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Visit Other APS Journals Online
Copyright © 1977 by the American Physiological Society.