| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Articles in PresS, published online ahead of print April 18, 2002
Am J Physiol Cell Physiol, 10.1152/ajpcell.00248.2001
Submitted on June 4, 2001
Accepted on April 16, 2002
1 Nutritional Sciences, University of Arizona, Tucson, Arizona, USA
2 Nutrition and Food Science, University of Maryland, College Park, Maryland, USA
* To whom correspondence should be addressed. E-mail: dl165{at}umail.umd.edu.
The objective of this work was to examine the importance of zinc status on the levels of p53, p21 and bcl-2 family gene expression, and caspase-3 activity in human aortic endothelial cells (HAECs). A serum-reduced low-zinc media (ZD) was used to deplete zinc over one passage. Other treatments included zinc-normal control (ZN) with 3.0 µmol/L of zinc for comparison with a zinc level normally found in most culture media, zinc-adequate (ZA) with 16.0 µmol/L of zinc for comparison to normal human plasma concentrations, and zinc-supplemented (ZS) with 32.0 µmol/L of zinc for comparison to human plasma zinc levels attained by oral supplementation. Cellular zinc levels in the ZD cells were 64% of ZN controls, and the ZA cells were not different but the ZS cells were significantly higher (40%) than ZN cells. No significant difference in p53 mRNA abundance was detected among all treatments, however, p53 protein levels were more than 100% higher in the ZD and ZS cells, and were almost 200% higher in the ZA cells as compared to ZN control levels. In contrast, p21 mRNA abundance, a downstream target of p53 protein, was significantly increased in the ZS cells as compared to both the ZN control and ZD cells. In the ZS cells, bax and mcl-1 were also approximately 50% higher as compared to ZN controls whereas bcl-2 mRNA was increased as compared to ZA cells. Moreover, caspase-3 activity of ZD cells was no different from ZN controls, but was reduced 83% in ZA and 69% in ZS cells. Thus, p53 protein, p53 downstream target genes, and bcl-2 family genes appeared to be modulated by intracellular zinc status in HAECs.
This article has been cited by other articles:
|
|
 |
|
  M. Yan, Y. Song, C. P. Wong, K. Hardin, and E. Ho Zinc Deficiency Alters DNA Damage Response Genes in Normal Human Prostate Epithelial Cells J. Nutr., April 1, 2008; 138(4): 667 - 673. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. H. K. Wong, Y. Zhao, N. W. Schoene, C.-T. Han, R. S. M. Shih, and K. Y. Lei Zinc deficiency depresses p21 gene expression: inhibition of cell cycle progression is independent of the decrease in p21 protein level in HepG2 cells Am J Physiol Cell Physiol, June 1, 2007; 292(6): C2175 - C2184. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. A. Alshatwi, C.-T. Han, N. W. Schoene, and K. Y. Lei Nuclear Accumulations of p53 and Mdm2 Are Accompanied by Reductions in c-Abl and p300 in Zinc-Depleted Human Hepatoblastoma Cells. Experimental Biology and Medicine, May 1, 2006; 231(5): 611 - 618. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Ho, C. Courtemanche, and B. N. Ames Zinc Deficiency Induces Oxidative DNA Damage and Increases P53 Expression in Human Lung Fibroblasts J. Nutr., August 1, 2003; 133(8): 2543 - 2548. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| Visit Other APS Journals Online |
